Dendrobium officinale propagation method capable of shortening vegetative propagation cycle of Dendrobium officinale

Abstract

The invention relates to a Dendrobium officinale propagation method which comprises the following steps: (1) adventitious bud induction: culturing a sterilized Dendrobium officinale stem section in an induction culture medium for 28-30 days, wherein the induction culture medium is composed of MS (Murashige-Skoog) basal culture medium, 0.1-0.15 mg / L naphthylacetic acid, 0.5-1.0 mg / L 6-benzylaminoadenine, 3 wt% sucrose and 0.8 wt% agar; (2) adventitious bud proliferation: after the induction is finished, cutting off the new bud growing at the internode of the stem section, inoculating into the induction culture medium to carry out subculture propagation for 30-32 days, and obtaining cluster buds at the cutting; and (3) sound seedling and root transplantation: cutting the cluster buds into single seedlings, transferring into a sound seedling culture medium to carry out sound seedling culture for 20 days, and carrying out rooting culture for 28 days after the buds develop into 2-3 blades which are 2-3cm high, wherein the sound seedling culture medium is composed of MS basal culture medium, 3 wt% sucrose, 0.7 wt% agar and 3 wt% organic fertilizer, and the rooting culture medium is composed of 1 / 2 MS basal culture medium, 0.3-0.5 mg / L naphthylacetic acid and 0.3-0.5 wt% activated carbon. The method reduces the pollution, lowers the cost, and enhances the survival rate of the tissue culture seedlings by more than 30%.

Description

technical field [0001] The invention relates to a cultivation technology of dendrobium candidum, in particular to a breeding method of dendrobium candidum capable of shortening the asexual reproduction cycle of dendrobium candidum. Background technique [0002] Dendrobium candidum, also known as black knot grass, commonly known as Tiepi Fengdou, is a perennial evergreen herb of the genus Dendrobium orchidaceae, and is a precious medicinal material. In recent years, studies have found that Dendrobium contains polysaccharides, alkaloids, phenanthrene and erianin and other compounds, as well as 7 kinds of essential amino acids and various trace elements. Modern pharmacology shows that Dendrobium has anti-tumor, anti-aging, and immune-enhancing functions, and has obvious therapeutic effects on cardiovascular, digestive system, respiratory system and ophthalmic diseases. Due to the medicinal value of Dendrobium candidum with many unique curative effects, it is called the "life-s...

AI Technical Summary

Problems solved by technology

[0003] In recent years, researchers have conducted a lot of research on the tissue culture rapid propagation technology of Dendrobium candidum, but they mainly focus on the proliferation and differentiation of protocorms, and there are not many studies on the rapid propagation of Dendrobium candidum in vitro using stem segments as materials.