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A kind of cultivation method of Dendrobium officinale

A technology of Dendrobium officinale and cultivation temperature, which is applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve many problems, and achieve the effects of reducing cost, improving survival rate and high induction rate.

Inactive Publication Date: 2016-07-13
铄洋生物科技(上海)股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, researchers have done a lot of research on tissue culture and rapid propagation of Dendrobium candidum, but they mainly focus on the proliferation and differentiation of protocorms.

Method used

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Embodiment Construction

[0012] The cultivation method of the Dendrobium officinale of the present embodiment may further comprise the steps:

[0013] (1) Adventitious bud induction: Take the young stems of Dendrobium candidum in the same year, cut them into small stems with a length of 1-1.5cm and 1-2 axillary buds, soak them in 75% alcohol for 30s after washing, and then Disinfect with 0.1% mercuric chloride for 10 minutes, and then rinse with sterile water for 5 times;

[0014] Cultivate the sterilized dendrobium stem section in the induction medium for 30d, the induction medium is: MS basic medium, 0.1mg / L naphthaleneacetic acid, 0.5mg / L 6-benzylaminoadenine, 3wt% sucrose, 0.8 wt% agar;

[0015] (2) Proliferation of adventitious buds: After the induction, cut off the new shoots that grow from the stem segments at the internodes, inoculate them in the induction medium for subculture and proliferate for 30 days, and obtain clustered buds at the cuts;

[0016] (3) Strong seedlings and rooting trans...

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Abstract

The invention relates to a cultivation method of Dendrobium officinale, comprising the following steps: (1) Inducing adventitious buds: culturing sterilized stem segments of Dendrobium in an induction medium for 28-30 days, the induction medium being: MS basic medium, 0.1-30 days 0.15mg / L naphthaleneacetic acid, 0.5-1.0mg / L 6-benzylaminoadenine, 3wt% sucrose, 0.8wt% agar; (2) Proliferation of adventitious buds: After the induction, the stem segments were grown at the internodes The new shoots are cut off, inoculated in the induction medium and subcultured for 30-32 days, and clustered shoots are obtained at the incision; (3) strong seedlings and rooted transplanting: cut the clustered shoots into single seedlings, and transfer them to the strong seedling medium Carry out strong seedling cultivation 20d, when bud development is high 2-3cm, and can carry out rooting culture when having 2-3 blades, rooting culture time is 28d; Wherein strong seedling culture medium is: B5 medium, 3wt% sucrose, 0.7wt% agar, 3wt% organic fertilizer, 15g / L mashed potatoes; rooting medium: 1 / 2MS basic medium, 0.3‑0.5mg / L naphthaleneacetic acid, 0.3‑0.5wt% activated carbon.

Description

technical field [0001] The invention relates to a cultivation technology of dendrobium candidum, in particular to a cultivation method of dendrobium candidum. Background technique [0002] Dendrobium candidum, also known as black knot grass, commonly known as Tiepi Fengdou, is a perennial evergreen herb of the genus Dendrobium orchidaceae, and is a precious medicinal material. In recent years, studies have found that Dendrobium contains polysaccharides, alkaloids, phenanthrene and erianin and other compounds, as well as 7 kinds of essential amino acids and various trace elements. Modern pharmacology shows that Dendrobium has anti-tumor, anti-aging, and immune-enhancing functions, and has obvious therapeutic effects on cardiovascular, digestive system, respiratory system and ophthalmic diseases. Because Dendrobium officinale has a variety of unique curative effects and medicinal value, the folks call it the life-saving fairy grass, and the medical circle calls it the giant p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 许维龙
Owner 铄洋生物科技(上海)股份有限公司
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