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A kind of biological bacterial agent for cotton growth promotion, anti-premature aging, anti-bell drop and its preparation method and application

A technology of strains and bacterial counts, applied in the fields of chemicals for biological control, botanical equipment and methods, plant growth regulators, etc., can solve the problems of slow growth, reduced cotton yield, premature cell senescence, etc. The effect of preventing shedding and easy use

Inactive Publication Date: 2016-08-17
XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the low temperature at the seedling stage of cotton in Xinjiang, the growth is slow. Generally, continuous spraying of gibberellin is used to solve this problem. However, the use of gibberellin will cause premature aging after excessive elongation of cells. In cotton During the boll-setting period, a large number of buds fall off, which can seriously reduce cotton production by more than 30%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of biological bacterial agent of the present invention is as follows:

[0027] 1. Preparation of the fermentation broth of three bacterial strains

[0028] (1) Preparation of the fermentation broth of the strain ACCC30352: prepare a liquid fermentation medium of the quality required for fermentation, insert the strain ACCC30352 into the liquid fermentation medium under sterile conditions, and shake and cultivate it on a shaker at 25°C for 72 hours After that, until the number of effective viable bacteria reached 2.0×10 9 seeds / ml to obtain the first-grade seeds; then, the cultivated first-grade seeds were inserted into the sterilized equipment (high-pressure steam sterilization method, the sterilization temperature was 121°C~125°C, the pressure 0.103Mpa~ 0.168Mpa, sterilization time 0.5h~1.0h.) and fermented in the fermenter of the cooled liquid fermentation medium, the fermentation temperature is 25°C, and the ventilation rate starts at 1:1.2 (v...

Embodiment 2

[0032] Example 2 Antagonism test

[0033] The strains ACCC30352, ACCC30334, and ACCC31052 were placed on PDA medium at three points with a distance of 3 cm, and each combination was repeated three times for cross-antagonism tests. The results showed that there was no antagonism between the three strains and they could grow together.

Embodiment 3

[0034] Embodiment 3 Determining the biological activity of the biological bacterial agent of the present invention

[0035] The bacterial agents ACCC30352, ACCC30334, ACCC31052 were used to compare the biological activity with the biological agent of the present invention, and water was used as a control to carry out the tests of promoting growth, anti-premature aging and anti-shedding. Among them, the growth-promoting test: the increase ratio of the height of the cotton seedlings compared with the above-mentioned bacterial agent and the clear water control; the anti-premature aging test: the incidence rate of the premature aging cotton plants compared with the above-mentioned bacterial agent; As shown in Table 1.

[0036] Table 1

[0037] .

[0038] As can be seen from the data in Table 1, the biological activity of the biological agent of the present invention is significantly better than that of a single biological bacterium, no matter in promoting growth, anti-prematur...

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PUM

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Abstract

The present invention provides a biological inoculant for cotton growth-promoting, anti-premature senescence, and anti-boll fall. The biological inoculum includes the following substances: strain ACCC30352, strain ACCC30334 and strain ACCC31052. The biological bacterial agent produced by the invention can promote the growth of cotton, solve the problem of slow growth of cotton seedlings in the early stage, and can resist premature senescence of cotton, improve the resistance and disease resistance of cotton, and can also protect flowers and protect It can prevent the shedding of cotton buds and bolls and significantly increase the yield of cotton. The biological inoculum of the present invention can be continuously sprayed (dropped) applied after the cotton emerges, and applied once every 7-10 days, and the effect of continuous use more than 5 times is remarkable. The biological bacterial agent of the invention is simple to use, convenient, low in cost, safe and environmentally friendly, and has high cost performance.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a biological bacterial agent used for cotton growth promotion, premature aging resistance and boll drop prevention. Background technique [0002] Due to the low temperature at the seedling stage of cotton in Xinjiang, the growth is slow. Generally, continuous spraying of gibberellin is used to solve this problem. However, the use of gibberellin will cause premature aging after excessive cell elongation. In cotton A large number of buds fall off during the boll formation period, which can seriously reduce cotton production by more than 30%. The leaders and researchers of the Agricultural Science Institute of the Sixth Agricultural Division have been researching and exploring the use of biotechnology and products to solve this problem for many years. Contents of the invention [0003] In order to solve the problems existing in the prior art, the pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P21/00A01N43/90A01N43/16A01N61/00
Inventor 贾晓军李东方任承才赵战胜张庭军邢晓东
Owner XINJIANG PRODION & CONSTR CORPS NO 6 DIV AGRI SCI INST
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