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Flow cell

A technology of flow chamber and liquid flow, which is applied in the field of flow chamber to achieve accurate absorbance measurement and suppress the effect of refractive index

Active Publication Date: 2014-03-12
SHIMADZU SEISAKUSHO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this structure is incompatible with the above-mentioned requirements of directly introducing measurement light into the light-introducing end of the capillary and emitting the light directly from the light-out-leading end of the capillary.

Method used

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Experimental program
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Embodiment Construction

[0041] Embodiments of the present invention will be described below with reference to the drawings.

[0042] image 3 It is a schematic cross-sectional view of the flow chamber 10 according to the embodiment of the present invention. Both ends of the capillary 1 made of fused silica are attached to the base body 3 in a liquid-tight manner by resin holding members 2 a and 2 b such as capillaries. A liquid introduction passage 4a leading to one end side of the capillary 1 and a liquid outlet passage 4b ​​leading to the other end side of the capillary 1 are formed on the base 3, so that the sample liquid is introduced into the capillary 1 through the liquid introduction passage 4a, so that The sample liquid flowing through the capillary 1 is discharged to the outside through the liquid outlet passage 4b.

[0043] A window member 5 a for light introduction is attached to one end side of the capillary 1 , and a window member 5 b for light introduction is attached to the other end...

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Abstract

The invention provides a flow cell, which can detour the abutting component of a maintain element at two ends of capillary with flowing sample liquid, and can conduct accurate light absorbance measuring through inhibiting the refractive rate effect without generating base line change. The flow cell includes holding members for holding two ends of a capillary. A refractive index of holding members is 1.31 or less or 1.40 or greater at least in portions through which the holding members make contact with an outer surface of the capillary. A numerical aperture of light for measurement that enters from a light source into the capillary is 0.22 or less so that reflectance of portions through which the holding members make contact with the capillary is constant even when sample liquid that flows through the capillary is converted to water and acetonitrile.

Description

technical field [0001] The present invention relates to a flow chamber for flowing a liquid to be measured, for example, when measuring the absorbance of the liquid or the like. Background technique [0002] In an absorbance detector used in a liquid chromatograph, etc., a liquid to be measured (hereinafter referred to as "sample liquid") is filled or continuously circulated in a container called a cell, and light from a light source is irradiated. To measure light, the intensity of light transmitted through the sample liquid is detected to obtain the absorbance for each wavelength. In order to measure a trace amount of sample liquid with high sensitivity, it is necessary to make the cross-sectional area of ​​the cell small and the optical path length long. Therefore, conventionally, a flow chamber called a "light pipe cell" or the like has been put into practical use. A linear capillary is used as the cell, and light is irradiated from one end side of the capillary along t...

Claims

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Application Information

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IPC IPC(8): G01N30/26G01N30/02
CPCG01N21/05G01N21/0303
Inventor 畠堀贵秀
Owner SHIMADZU SEISAKUSHO CO LTD
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