Biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus
A technology of black-streaked dwarf virus and black-streaked dwarf disease, which is applied in the field of in vivo biological detection, can solve the problems of high and low virus transmission rate, plant infection and other problems, shorten the test period, achieve accurate and reliable results, and eliminate the source of external virus-transmitting insects interference effect
Inactive Publication Date: 2014-03-19
ZHEJIANG JIAXING AGRI SCI ACADEMY INST
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Problems solved by technology
At present, there is no complete and practical detection method for the transmission rate of the virus after SBPH is infected, and the interval between plant infection and symptoms.
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[0015] The present invention will be further described below in conjunction with examples, but the embodiments of the present invention are not limited thereto.
[0016] Example.
[0017] see figure 1 , the biological detection method of SBPH of the present invention to the transmission rate of black-streaked dwarf virus, mainly consists of the following steps:
[0018] (1) Feeding poison: Collect black-streaked dwarf diseased plants and cultivate them intensively in turnover boxes with a length × width × height of 50cm × 45cm × 30cm. Plant 9 plants in each turnover box and move the turnover box to the insect-proof net room , the size of the insect-proof net in the insect-proof net room is 80 mesh, and the length × width × height of the insect-proof net room is 50cm × 45cm × 70cm. The diseased plants and the normal growth environment of SBPH can be fed continuously for 7 days. The insect-proof net room can prevent the migration of SBPH from the outside and the escape of SBPH...
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Abstract
The invention relates to a living-body biological detection method, and specifically relates to a biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus. The detection method comprises: breeding laodelphax striatellus on intensively cultivated black-streaked dwarf viral disease plants, after 7 days, transferring laodelphax striatellus forcedly bred with virus to healthy disease-free rice seedlings in the three-leaf-and-one-leaflet stage and enabling laodelphax striatellus to feed for 48 h, then transplanting eaten price seedlings to an observation garden, observing the occurrence time and the occurrence number of black-streaked dwarf viral disease, and obtaining the black-streaked dwarf virus transmission rate of laodelphax striatellus and the incubation period of the virus in the plant by statistics. The invention aims at providing an accurate, reliable, short-period scientific effective biological detection method for black-streaked dwarf virus transmission rate of laodelphax striatellus.
Description
technical field [0001] The invention relates to a biological detection method in vivo, in particular to a detection method for the transmission rate of the black-streaked dwarf virus by the small planthopper. Background technique [0002] Rice black-streaked dwarf disease is a viral disease that causes rice plant dwarf, and the virus is mainly transmitted by the small planthopper. After feeding on the diseased plants, the small planthoppers will transfer the virus to the healthy plants. At present, there is no complete and practical detection method for the transmission rate of the virus after SBPH is infected, and the interval between plant infection and symptoms. Contents of the invention [0003] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art, and provide an accurate, reliable, short cycle, scientific and effective biological detection method for the transmission rate of SBPH to black-streaked dwarf virus. [0004...
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IPC IPC(8): C12Q1/70
CPCA01G7/06
Inventor 曹奎荣孙祥良
Owner ZHEJIANG JIAXING AGRI SCI ACADEMY INST