Actinomyces Israeli production technology, Actinomyces Israeli preparation and Actinomyces Israeli production apparatus

A nematicidal fungus and production process technology, applied in the field of nematicidal fungus production process and its preparation and production device, can solve the problems of unsuitable temperature control, products contaminated by miscellaneous bacteria, solid fermentation failure and the like, and achieve considerable effects , Prevent bacterial contamination, and facilitate the effect of thorough sterilization

Inactive Publication Date: 2014-03-26
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the solid fermentation process of nematicidal fungus ehrlichii, the temperature has a great influence on it. Properly raising the temperature is beneficial to the solid fermentation of nematicidal fungus ehrlichii and can increase the amount of spore production, but the heat release of solid fermentation process, it is easy to cause the internal heat of the solid medium to exceed 31 degrees, and it is easy to burn the colony to death, which is not conducive to sporulation
The nematocide fungus is aerobic, so increasing the ventilation in an appropriate amount will help to increase the growth rate of the spores, but the ventilation rate should not be too large during solid fermentation, because the growth rate will be faster, the internal heat will increase, and the tempe

Method used

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  • Actinomyces Israeli production technology, Actinomyces Israeli preparation and Actinomyces Israeli production apparatus
  • Actinomyces Israeli production technology, Actinomyces Israeli preparation and Actinomyces Israeli production apparatus
  • Actinomyces Israeli production technology, Actinomyces Israeli preparation and Actinomyces Israeli production apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] a) Preparation work, clean and sterilize the operation room, ultra-clean workbench, and fermentation tank, wipe the ultra-clean table and fermentation tank with 75% ethanol, and then irradiate the three with UV for 2 hours; put all sterilized equipment directly The ultra-clean workbench is cooled to below 30 degrees, during which the ultraviolet lamp and blast are turned on.

[0080] Operators who enter the operation room perform thorough disinfection by themselves.

[0081] b) Preparation of Evansi-killing fungus strains: ingredients according to the following components and weight percentages: potato 10%, glucose 2%, wheat bran 0.5%, remaining water; put all the ingredients into a triangular flask, and conventional high temperature and high pressure sterilization After 30 minutes, the colony scraped from the plate was inserted after cooling, placed on a shaker, the ambient temperature was kept at 26 degrees, the rotating speed of the shaker was 180 rpm, and the culture tim...

Embodiment 2

[0091] Embodiment 2: Method steps a to f are the same as in embodiment 1, and then:

[0092] h) Prepare 3-5% sodium alginate solution, clay, 0.1M sodium citrate solution, 0.2M calcium chloride solution, rubber stopper glass bottle, and perform routine sterilization;

[0093] i) Add 10 times water to the cultured solid carrier medium, filter with 100 mesh nylon yarn, add an equal volume of 3-5% sodium alginate solution to the filtrate obtained after filtration, add 10% clay, and press Add penicillin at a ratio of 0.05 mg / ml, add streptomycin at a ratio of 0.025 mg / ml, stir evenly, put it into a rubber stopper glass bottle, cover the rubber stopper, and pass the plastic tip with a rubber tube through the rubber stopper Insert the bottle into the bottle, place a beaker containing 0.2M calcium chloride solution under the other end of the rubber tube, and install a control valve on the rubber tube to make the liquid in the bottle drip into the beaker continuously to form small spherical...

Embodiment 3

[0096] The method steps a to g are similar to the embodiment 1, the difference lies in:

[0097] In step c, the liquid culture medium is prepared according to the following components and weight percentages: peptone 3%, glucose 1.5%, yeast powder 1.5%, antifoaming agent 0.15%, water 91.85-97.17%.

[0098] In step d, the solid medium is prepared according to the following components and weight percentages: 15% wheat bran, 30% pine wood chips, and 65% water.

[0099] What can also be obtained by the above-mentioned method is the semi-finished product of the Evandella fungal preparation.

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PUM

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Abstract

The invention discloses an Actinomyces Israeli production technology, an Actinomyces Israeli preparation and an Actinomyces Israeli production apparatus. The production technology adopts a liquid-solid biphasic cultivation method to realize the mass production of Actinomyces Israeli, liquid fermentation is carried out to obtain a large amount of mycelia and blastospores and a small amount of conidiospores, and the above obtained substances are transferred to a solid cultivation medium to generate a large amount of aerial conidiospores. An Actinomyces Israeli pill or pulvis prepared through the technology is convenient to store, and can be dissolved in a 0.1M sodium citrate solution or is diluted by adding water when the pill or pulvis is used. The production apparatus comprises a disc body, the base plate of the disc body is punched with a plurality of holes, and at least one metal screen covering all the holes is fixed on the base plate. The apparatus guarantees the oxygen and humidity required by the solid cultivation of the Actinomyces Israeli, effectively prevents the living contaminants, and substantially improves the sporulation quantity of the Actinomyces Israeli.

Description

Technical field [0001] The present invention belongs to the technical field of microbial insecticides, and in particular relates to a production process of Evangelion fungus and its preparation and production device. Background technique [0002] Pine wood nematode disease is also called pine wilt disease. It is a devastating epidemic of pine trees. It has strong pathogenicity, with many transmission routes, hidden disease sites, and fast host death; it spreads quickly and is often caught off guard; once it occurs, it is difficult to manage. Called the cancer of the pine tree. According to incomplete statistics, in the 10 years after the occurrence of the disease in my country in 1982, the area has reached approximately 38,000 ha. 2 , Causing the death of more than 1,400,000 pine trees and the loss of 50,000m of wood 3 . The funds for disease prevention and control reached 6.45 million yuan. It not only causes huge losses to the national economy, but also destroys the natural...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12M1/00A01N63/04A01P5/00
Inventor 张永安曲良建王青华薛建杰王春燕王振王玉珠杜婷
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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