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Protein pattern identification method based on microcantilever platform

A micro-cantilever beam and pattern recognition technology, applied in the field of biosensors, can solve the problems of limited application, limited detection of substances, failure to identify various proteins, etc., and achieve the effect of fast detection speed and easy operation

Inactive Publication Date: 2014-03-26
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, the research on the microcantilever has stayed at the performance stage of the instrument itself, and its application is far from being used to identify multiple proteins. There are only simple single-substance detection, and the application is not widespread. There are many Large limitations, detection substances are mostly limited by known specific reactions

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  • Protein pattern identification method based on microcantilever platform
  • Protein pattern identification method based on microcantilever platform
  • Protein pattern identification method based on microcantilever platform

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A single-sided gold-plated micro-cantilever probe was used to immobilize six sulfhydryl compounds with different chemical groups on the gold-plated surface of the micro-cantilever probe by using the chemical bonds between gold and sulfhydryl groups. The six substances are: (3-mercaptopropyl)trimethoxysilane, 11-mercapto-1-undecyl alcohol, benzyl mercaptan, 1-dodecanethiol, 11-mercaptoundecanoic acid, 2- Dimethylaminoethanethiol hydrochloride.

[0032] First place the micro-cantilever beam probe in absolute ethanol for 3 minutes, and then irradiate it with ultraviolet light for 5 minutes to sterilize it. The probe is fixed on the modified sample stage, and each beam is immersed in one end of the capillary, and the other end of the capillary is immersed in the solution to be modified. Using the siphon effect of the capillary, different cantilever beams are modified with different sulfhydryl compounds. The thiol compound was dissolved in ethanol solution with a concentrat...

Embodiment 2

[0037] A single-sided gold-plated micro-cantilever probe was used to immobilize six sulfhydryl compounds with different chemical groups on the gold-plated surface of the micro-cantilever probe by using the chemical bonds between gold and sulfhydryl groups. The six substances are: (3-mercaptopropyl)trimethoxysilane, 11-mercapto-1-undecyl alcohol, benzyl mercaptan, 1-dodecanethiol, 11-mercaptoundecanoic acid, 2- Dimethylaminoethanethiol hydrochloride.

[0038] First place the micro-cantilever beam probe in absolute ethanol for 3 minutes, and then irradiate it with ultraviolet light for 5 minutes to sterilize it. The probe is fixed on the modified sample stage, and each beam is immersed in one end of the capillary, and the other end of the capillary is immersed in the solution to be modified. Using the siphon effect of the capillary, different cantilever beams are modified with different sulfhydryl compounds. The thiol compound was dissolved in ethanol solution with a concentrat...

Embodiment 3

[0043] A single-sided gold-plated micro-cantilever probe was used to immobilize six sulfhydryl compounds with different chemical groups on the gold-plated surface of the micro-cantilever probe by using the chemical bonds between gold and sulfhydryl groups. The six substances are: (3-mercaptopropyl)trimethoxysilane, 11-mercapto-1-undecyl alcohol, benzyl mercaptan, 1-dodecanethiol, 11-mercaptoundecanoic acid, 2- Dimethylaminoethanethiol hydrochloride.

[0044] First place the micro-cantilever beam probe in absolute ethanol for 3 minutes, and then irradiate it with ultraviolet light for 5 minutes to sterilize it. The probe is fixed on the modified sample stage, and each beam is immersed in one end of the capillary, and the other end of the capillary is immersed in the solution to be modified. Using the siphon effect of the capillary, different cantilever beams are modified with different sulfhydryl compounds. The thiol compound was dissolved in ethanol solution with a concentrat...

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Abstract

The invention relates to a protein pattern identification method based on a microcantilever technology. Chemical compounds with different chemical groups are modified on the surface of the microcantilever and are used as a sensor array. Proteins to be measured are contacted and reacted with the array. The intermolecular reaction force between the different proteins and the sensor array is different, and therefore the distance deviated from the center of the microcantilever is different, and a group of fingerprint data is obtained by measuring the distance, and can identify the different proteins. The method utilizes a linear discriminant analysis method to analyze a plurality of the groups of the fingerprint data so as to prove that the different proteins can be identified by the identification method. The identification method is advantageous in that: no label or external probe is needed to be modified on molecules, biomacromolecules are not influenced by any labels, the nature quality of the biomacromolecules is completely remained, operation is simple, the reaction comprises only one step, and identification is rapid.

Description

technical field [0001] The present invention is a protein recognition method based on the micro-cantilever platform, adopts the pattern recognition technology, utilizes the change of the micro-cantilever signal caused by the reaction between protein molecules and different chemical groups, so as to achieve the purpose of distinguishing different kinds of proteins . The invention belongs to the field of biosensors. Background technique [0002] Changes in the content of certain specific proteins in the body are often closely related to the occurrence and development of certain diseases. Therefore, a protein recognition sensor with high sensitivity and high accuracy will provide greater convenience for early diagnosis of diseases. In protein recognition, in addition to the conventional "lock and key model" recognition, pattern recognition has great potential in the field of sensors. The way of pattern recognition originally benefited from bionics, simulating the olfactory sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803
Inventor 王萍樊春海金彩虹何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH