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Method for improving characteristics of wild broussonetia papyrifera through germ cell culture

A technology of somatic cells and germs, which is applied in the field of cultivating and improving wild tree traits by using embryo somatic cells, can solve the problems of increased production costs, poor continuity of excellent biological traits, and unsustainable biological traits, etc., to achieve wood fiber growth, long-term Enhanced effect of wood fiber and fast growth

Inactive Publication Date: 2014-04-02
云南晋企生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method not only increases the production cost, but also the biological traits cannot be continued
[0004] In recent years, it has been reported that people use plant somatic cells for tissue culture to improve plant biological traits; but because of the high separation of subculture, the continuity of excellent biological traits is not strong

Method used

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  • Method for improving characteristics of wild broussonetia papyrifera through germ cell culture
  • Method for improving characteristics of wild broussonetia papyrifera through germ cell culture
  • Method for improving characteristics of wild broussonetia papyrifera through germ cell culture

Examples

Experimental program
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Effect test

Embodiment 1

[0048] 1) Use wild paper mulberry as the female parent: choose a thicker wild paper paper;

[0049] 2) Germ selection and cutting: Select the full-bodied wild mulberry seeds from the wild mulberry tree selected in step 1), germinate under soilless conditions, and cut the middle germ when the germ grows to 1.5 cm, with a length of 1.8 cm. cm; Embryo somatic cells refer to the explants used for culturing somatic cell tissue culture, and are the life basis of improved mulberry trees. From the perspective of plant physiology, the germ is a living plant holographic cell with the ability to differentiate organs such as roots, stems, and leaves. It can be cultivated by related biotechnology to form plants, and it is a basic plant with physiological activities such as growth and branching; The selection directly affects the rate of callus emergence, so it is the key to select strong and white embryos;

[0050] 3) Sterilization of germs: immerse the germs cut in step 2) in 75% alcohol...

Embodiment 2

[0076] 1) Use wild paper mulberry as the female parent: choose a thicker wild paper paper;

[0077] 2) Germ selection and cutting: Select the full-bodied wild mulberry seeds from the wild mulberry tree selected in step 1), germinate under soilless conditions, and cut the middle germ when the germ grows to 2cm, with a length of 1cm; Embryo somatic cells refer to the explants used for culture in somatic cell tissue culture, and are the life basis of improved mulberry trees. From the perspective of plant physiology, the germ is a living plant holographic cell with the ability to differentiate organs such as roots, stems, and leaves. It can be cultivated by related biotechnology to form plants, and it is a basic plant with physiological activities such as growth and branching; The selection directly affects the rate of callus emergence, so it is the key to select strong and white embryos;

[0078] 3) Sterilization of germs: immerse the germs cut in step 2) in 75% alcohol, disinfe...

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Abstract

The invention discloses a method for improving the characteristics of wild broussonetia papyrifera through germ cell culture. The method comprises the steps of (1) taking wild broussonetia papyrifera as female parent; (2) selecting and slicing germ; (3) sterilizing the germ; (4) inducing callus; (5) promoting differentiation of the callus into seedling to obtain a complete plant; and (6) managing after differentiating into seedling. The method disclosed by the invention, on the basis of germ cell tissue culture technology, enhances fast growth performance of the wild broussonetia papyrifera, lengthens wood fiber and increases the content of plant protein in tender shoots and leaves, so that the wild broussonetia papyrifera can grow at ultra-fast speed and have long wood fiber, and the tender shoots and leaves are rich in nutrition.

Description

technical field [0001] The invention relates to a method for improving the traits of wild tree mulberry by cultivating embryo somatic cells. Background technique [0002] Broussonetia papyrifera, scientific name (Broussonetia papyrifera), is a perennial, deciduous tree of the genus Moraceae. Its stems are white and can be used as raw materials for papermaking. The shoots and leaves contain plant protein and can be used as animal feed. However, due to the short length of wood fibers, High-quality pulp cannot be produced. Therefore how to cultivate the longer mulberry plant of wood fiber has just become a subject of people's exploration. [0003] For a long time, the majority of producers mainly use methods such as fertilization, removal of lateral buds, and some even use plant hormones such as gibberellin to achieve the purpose of cultivating so-called longer wood fibers and fast growth. This method not only increases the production cost, but also the biological traits...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈民方显初李勇鄯保玉李军
Owner 云南晋企生物科技有限公司
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