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A method for solid-state culture of microorganisms using a solid-state culture device for microorganisms

A solid-state culture and microorganism technology, applied in the direction of microorganism-based methods, methods of supporting/immobilizing microorganisms, enzymology/microbiology devices, etc., can solve the problems of unfavorable microbial spore growth and achieve high efficiency

Active Publication Date: 2015-12-09
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this automatic rotary microbial spore incubator, the material is piled up at the bottom of the incubator, which makes the gradient difference of temperature and humidity between the upper and lower layers of the material layer large, which is not conducive to the growth of microbial spores.

Method used

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  • A method for solid-state culture of microorganisms using a solid-state culture device for microorganisms
  • A method for solid-state culture of microorganisms using a solid-state culture device for microorganisms
  • A method for solid-state culture of microorganisms using a solid-state culture device for microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] use figure 2 The shown microbial solid-state culture device carries out microbial solid-state culture, that is, the microorganism solid-state culture device includes a box body 10, a first inlet pipe 20, a second inlet pipe 23, a discharge pipe 30, a sieve plate 40 with sieve holes, and a main pipe 50. , a steam pipe 60 and an air pipe 70 communicating with the main pipe 50 at the upstream of the main pipe 50, the box body 10 includes a box main body 11 and a jacket 12, the sieve plate 40 is arranged on the outer wall of the box main body 11 and Formed as a part of the outer wall, a jacket 12 is provided on the box main body 11 corresponding to the sieve plate 40, and there is a gap 13 between the outer wall of the box main body 11 and the outer wall of the jacket 12, and the gap 13 The heating element of the heating device is arranged in the middle, the interval 13 between the sieve plate 40 and the outer wall of the jacket forms the lower layer, one end of the first ...

Embodiment 2

[0084] The solid-state culture of Aspergillus niger was carried out according to the method of Example 1, except that the microorganism solid-state culture device did not include the second inlet pipe 23, and the results are shown in Table 1.

[0085] Table 1

[0086] Example

Spore quantity (pcs / g material)

The number of miscellaneous bacteria (a / g material)

Acid production level (g / 100mL)

Example 1

1.01 billion

10

13.8

Example 2

980 million

35

13.4

[0087] It can be seen from the results in Table 1 that the solid-state culture method of the present invention has higher culture efficiency and is suitable for industrial application.

[0088] After the sieve plate is set at the bottom of the box, sterile gas or water vapor is introduced into the box from the bottom of the box, and the sterile gas or water vapor rises and passes through the materials accumulated on the sieve plate, so that the upper layer of the m...

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Abstract

The invention discloses a method for performing microbial solid culture by using a microbial solid culture device. The device comprises a box body, a first inlet pipe, an outlet pipe and a sieve plate with sieve pores, wherein the sieve plate is arranged in the box body and separates the inner space of the box body into an upper layer and a lower layer; the first inlet pipe is communicated with the lower layer of the box body; the box body is communicated with the outside through the outlet pipe. The method comprises the following steps: introducing steam to sterilize a material in the box body, inoculating a microbial fermentation seed to be cultured into the box body, enabling the microbial fermentation seed to be in contact with the material, and culturing; in the culturing process, introducing sterile gas into the box body, wherein the introduced steam or sterile gas enters the upper layer of the box body through the first inlet pipe and the sieve pores in the sieve plate to be in contact with the material or the material inoculated with the fermentation seed, the gas subjected to contact is discharged from the box body through the outlet pipe, and the material can not pass through the sieve pores. According to the invention, the microbial solid culture efficiency is high.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a method for solid-state cultivation of microorganisms using a solid-state cultivation device for microorganisms. Background technique [0002] Solid-state fermentation is one of the technologies with the longest history for humans to use microorganisms to produce products. The brewing industry with a long history in my country is a typical representative of solid-state fermentation. Solid-state fermentation contains water-insoluble solids, a small amount of water and air. The heat generated by microorganisms causes water to evaporate, making the fermentation system have a three-phase inhomogeneous vapor-liquid-solid, with serious concentration gradients and difficulties in heat and mass transfer. It is difficult to control pH, water activity, optimal reaction temperature, etc., which greatly reduces the yield, especially the first condition of modern fermentation techno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/12C12M1/04C12M1/02C12M1/36C12M1/38C12N1/14C12R1/685
CPCC12M21/16C12M23/34C12M29/04C12M41/20C12M41/34C12N1/14
Inventor 周勇卢宗梅钟华刘梦涵
Owner COFCO BIOTECHNOLOGY CO LTD