Nitrogen source, culture medium and culture method for producing riboflavin

A riboflavin and culture medium technology, which is applied in the field of biological production of riboflavin, can solve the problems of excessive bacterial protein salt, no utilization of bacterial protein, low added value, etc., so as to promote bacterial growth and realize resource utilization. The effect of recycling, reduced use

Active Publication Date: 2014-04-02
GUANGJI PHARMA MENGZHOU
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AI Technical Summary

Problems solved by technology

Although this method provides a way to use waste cells as a nitrogen source, it does not use the bacterial protein with high salinity, nor does it combine

Method used

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Embodiment

[0021] Put the seed culture medium into a 10 m3 seed tank. After the disinfection is completed, insert the prepared Bacillus subtilis spore suspension and cultivate it aerobically at 120-140 rpm for 12-16 hours at 37-39°C. Under the premise that the mycelium of the seed solution is divided, the microscopic examination is normal, there is no miscellaneous bacteria, and all the test data and indicators meet the process requirements, the connecting pipeline from the seed tank to the fermenter is cleaned up, and the transplant is ready.

[0022] Use a pump to pump the fermentation medium into a 200m3 fermenter through the connecting pipeline and magnetization device, and then add sterile glucose solution after disinfection and sterilization to make the initial sugar concentration of the medium in the fermenter reach 15g / L , adjust the pH to 6.7-7.1 with liquid ammonia, and control the ventilation ratio (VVM) to 1:0.6. Put in the cultured normal seed liquid, control the fermentatio...

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Abstract

The invention relates to a method for obtaining a nitrogen source for producing riboflavin. The method comprises the following steps: performing desalination treatment on wastewater of a riboflavin fermentation broth after extraction, wherein the total salt content of the wastewater is controlled to be less than 5g/l after desalination; adding 2-5% of polymerized ferrous sulfate for flocculating for 1-2 hours, centrifuging for filtering by using a double-conical decanter centrifuge so as to obtain a solid substance, and drying the solid substance so as to obtain bacterial protein. By adopting the method, the problem that the riboflavin bacterial protein is hard to utilize because of high salt content is solved, the bacterial protein which can be alternatively used as a fermentation nitrogen source is obtained, the application of the expensive organic nitrogen source is greatly reduced, and recycling and utilization of resource are achieved. Besides, on the basis of the bacterial protein, the invention further discloses a corresponding seed culture medium formula and a fermentation culture medium formula, and the process control conditions are optimized starting from factors including temperature, pH values, residual sugar content, dissolved oxygen content, ammonia feeding amount, material supplement mode and the like which can affect the stability of fermentation.

Description

technical field [0001] The invention relates to the production of riboflavin by a biological method. Background technique [0002] The main carbon source used in the microbial fermentation of riboflavin is vegetable oil, glucose, molasses or rice sugar, etc.; the organic nitrogen source is mainly peptone, bone glue, fish meal, corn steep liquor and well-proportioned biological nitrogen; the inorganic salts include NaCl, K2HPO4, MgSO4, etc. The prices of the raw materials used are constantly rising, leading to high costs in industrial applications. At the same time, the production wastewater in the riboflavin fermentation industry is high-concentration organic wastewater, which has the characteristics of high chroma, high salinity, high COD, high BOD, and high bacterial content, and the treatment is difficult. Wastewater contains a large amount of sugar, protein, SS and ammonia nitrogen. These substances are not toxic and harmful substances, and can be used by some microorg...

Claims

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Application Information

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IPC IPC(8): C12P25/00C12N1/20C12R1/125
Inventor 王专旭高峰林建华周秀桃
Owner GUANGJI PHARMA MENGZHOU
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