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Method for detecting safety of exogenous genes in transgenic crops on soil nematodes

A technology of exogenous genes and soil nematodes, applied in the field of molecular biology, to achieve the effect of easy breeding of nematodes and simple methods

Inactive Publication Date: 2014-04-02
PEKING UNIV
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Problems solved by technology

The off-target effect of this exogenous gene on soil nematodes is the missing link that was not monitored in previous studies

Method used

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  • Method for detecting safety of exogenous genes in transgenic crops on soil nematodes
  • Method for detecting safety of exogenous genes in transgenic crops on soil nematodes
  • Method for detecting safety of exogenous genes in transgenic crops on soil nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Effect of exogenous genes cry1A and cpti in bivalent transgenic cotton on Caenorhabditis elegans

[0054] 1. Bioinformatics analysis of sequences

[0055] According to the patent information of bivalent transgenic cotton (Guo Sandui et al., 1999), the sequence information of the transferred exogenous genes cry1A and cpti was obtained.

[0056] Compare and analyze the acquired exogenous gene cry1A and cpti sequences with the whole genome sequence of Caenorhabditis elegans using the BLAT program (http: / / genome.ucsc.edu / index.html) to find out the complete match and Target gene fragments with a length of 17 bp or more.

[0057] These short fragments were searched and analyzed in Wormbase (http: / / www.wormbase.org / ) to find out the sequence of the target gene fragments that can reversely complement the exons in the Caenorhabditis elegans genome. The results of bioinformatics analysis of the sequences are shown in Table 1.

[0058] Table 1 Comparison results of e...

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Abstract

The invention provides a method for detecting the safety of exogenous genes in transgenic crops on soil nematodes. The method comprises the following steps: obtaining identical short fragments of the exogenous genes in the transgenic crops and genomes in caenorhabditis elegans through bioinformatic analysis, feeding the caenorhabditis elegans by cloning and converting the short fragments, and detecting the safety of the exogenous genes on the nematodes according to the phenotypic difference of the caenorhabditis elegans. In the past, the safety evaluation on the transgenic crops focuses on the influence of feeding foreign protein on non-target organisms. The method is used for detecting the safety of the exogenous genes on the nematodes which are important framers of soil eco-systems, and filling the blank of the safety evaluation field of the transgenic crops. The method has the advantages that RNAi (Ribonucleic Acid interference) is carried out by adopting a feeding method, special reagents and instruments are not needed, nematode feeding is easy, and the method is simple and practicable.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for detecting the safety of exogenous genes of transgenic crops to soil nematodes. Background technique [0002] It has been 16 years since the large-scale planting of genetically modified crops in 1996. The large-scale release of genetically modified crops in the environment has made the safety of genetically modified crops more and more the focus of attention, and has caused many controversy. The risk assessment of genetically modified crops has been carried out along with the research of genetically modified crops, and has always been an important part of the licensing of genetically modified crops. According to Directive 2001 / 18 / EC issued by the European Parliament in 2001, the purpose of the risk assessment of genetically modified crops is to identify and evaluate the possible direct or indirect, short-term or long-term negative impacts of genetically modified crop...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/02C12R1/19
CPCA01K67/0336A01K2227/703C12N15/102
Inventor 许崇任王戎疆李家练李茹
Owner PEKING UNIV
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