Method for inducing dc-cik by cell wall dipeptide
A technology of DC-CIK and MDP-DC-CIK, applied in the field of cell wall dipeptide, can solve the problem of high tumor cell killing activity, achieve the effect of improving tumor killing activity, promoting T cell differentiation and reducing tumor load
Active Publication Date: 2016-04-20
中海峡福建细胞生物科技有限公司
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[0031] However, the current conventionally induced and cultured DC-CIK cells are not very active in killing tumor cells, which requires a new induction method
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[0051] Preferred embodiments of the present invention will be described in detail below.
[0052] 1. Synthesis of muramyl dipeptide (MDP)
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The invention provides a method for inducing DC-CIK by utilizing muramyl dipeptide, that is to say, the muramyl dipeptide is added into a CIK or DC-CIK culture solution to induce proliferation and differentiation of CIK or DC-CIK cells, so as to improve the tumor-killing activity of the CIK or DC-CIK cells. The method comprises the following steps: peripheral blood collection, tumor antigen acquisition, mononuclear cell separation, mononuclear cell collection, mononuclear cell washing, MDP-DC-CIK cell induction, and culture. MDP-DC-CIK cells introduced and cultured by the method are detected by a flow cytometry, the ratio of CD3+CD56+ non-MHC restricted NKT cells is found to be up to 80% or more; and at the same time, the activity of tumor killer cells is much higher than that of DC-CIK cells by conventional induction culture, and a laboratory test result of the tumor-killing percentage reaches 99% or more.
Description
technical field [0001] The invention relates to the field of immune technology, in particular to the cell wall dipeptide obtained by protein polypeptide synthesis technology, which is added to the induction medium to induce the proliferation and differentiation of MDP-DC-CIK cells, so that the tumor-killing activity of MDP-DC-CIK is greatly improved Methods. Background technique [0002] Tumor cell biotherapy is an emerging tumor treatment mode with significant curative effect, and it is a new treatment method for autologous immune cells to fight cancer. It is a method of inducing, culturing and amplifying immune cells collected from tumor patients in vitro by using biotechnology and preparations, and then infusing them back into the patient's body. By stimulating, regulating and enhancing the body's autoimmune function, the purpose of treating tumors is achieved. . Tumor biological therapy is another tumor treatment technology after surgery, radiotherapy and chemotherapy,...
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IPC IPC(8): C12N5/0784C12N5/0783A61K35/15A61K35/17A61P35/00
Inventor 吴炯张炬
Owner 中海峡福建细胞生物科技有限公司