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Poloxamer gel adjuvant for O-typed foot and mouth disease polypeptides

A technology for poloxamer gel and foot-and-mouth disease, which is applied in the directions of antiviral agents, aerosol delivery, medical preparations containing active ingredients, etc. Achieve the effect of prolonging the release time of the polypeptide, improving the immune titer, good biocompatibility and safety

Inactive Publication Date: 2014-04-30
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overall, although oil-emulsion adjuvants have the function of enhancing the immune activity of vaccines, they have large toxic and side effects, are highly irritating to the body, and are likely to cause inflammatory reactions and other adverse reactions, which need to be improved.

Method used

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  • Poloxamer gel adjuvant for O-typed foot and mouth disease polypeptides
  • Poloxamer gel adjuvant for O-typed foot and mouth disease polypeptides
  • Poloxamer gel adjuvant for O-typed foot and mouth disease polypeptides

Examples

Experimental program
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Effect test

Embodiment Construction

[0018] The present invention will be further described in detail below in combination with specific embodiments.

[0019] O-type foot-and-mouth disease peptide antigen (provided by Shenlian Biomedical (Shanghai) Co., Ltd.), concentration 7.88 mg / mL; Poloxamer 407 and Poloxamer 188 (purchased from BASF (China) Co., Ltd.).

[0020] The amount of raw materials used in the preparation of poloxamer gel adjuvant in Examples 1 to 5 is shown in Table 1:

[0021] Table 1

[0022]

Example 1

Example 2

Example 3

Example 4

Example 5

Poloxamer 407 / g

1.9

2.3

2.0

2.1

2.2

Poloxamer 188 / g

0.1

0.8

0.3

0.5

0.6

Deionized water / mL

10

10

10

10

10

Critical gelling temperature / ℃

34.8

34.9

34.9

34.2

35.0

37℃ gel viscosity / Pas

794.3

422.1

526.4

628.7

798.6

Gel viscosity at 25℃ / Pas

21.31

0.111

12.17

24.59

21.71

...

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Abstract

The invention relates to a poloxamer gel adjuvant for O-typed foot and mouth disease polypeptides and a preparation method of the gel adjuvant. The gel adjuvant comprises 19-23 g of poloxamer 407, 1-8 g of poloxamer 188 and water. The preparation method comprises the following steps: adding part of deionized water into the poloxamer 407 and the poloxamer 188; refrigerating and stilling for complete dissolution; adding the rest deionized water; before using, adding O-typed foot and mouth disease polypeptide antigens till the concentration reaches 50 microgram / ml; shaking up. The poloxamer gel adjuvant prepared according to the invention has high immunological properties and good extracorporeal slow releasing properties.

Description

technical field [0001] The invention relates to a poloxamer gel adjuvant of O-type foot-and-mouth disease polypeptide, belonging to the field of pharmaceuticals. Background technique [0002] Poloxamer is a kind of PEO-PPO-PEO type nonionic triblock copolymer composed of polyoxyethylene (PEO) and polyoxypropylene (PPO). There are Pluronic, Synperonic or Tetronic. When the concentration of the poloxamer aqueous solution is above a certain critical value, as the temperature rises, sol-gel (low-temperature sol-gel boundary) and gel-sol (high-temperature gel-sol boundary) appear The transition of the gel phase occurs between the two critical transition temperatures, and the transition temperature varies with the composition and concentration of the polymer. [0003] Poloxamer thermosensitive gel has many advantages, including good biocompatibility, compliance, sustained and controlled release ability, and high safety. The acute toxicity test of Poloxamer 188 shows that its LD5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/06A61P31/14A61K39/135
Inventor 王霄旸王昊欣张可煜薛飞群张丽芳费陈忠王米
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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