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Single sample nucleic acid enclosed extractor

A closed, single-sample technology, used in biochemical instruments, biochemical equipment and methods, DNA preparation, etc., can solve the problems of large volume, large amount of consumables, high cost, avoid cross-contamination, avoid interference, and improve extraction efficiency. Effect

Active Publication Date: 2014-04-30
XIAN TIANLONG SCI & TECH
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] At present, most of the automatic nucleic acid extractors use the magnetic bead method to extract nucleic acid. During the extraction process, the test tube mouth is open, and they are all test tube groups designed for multi-sample extraction. The test tube group is an open extraction The test tubes on the platform and the test tube set are not covered with test tube covers, which is easy to cross-contaminate during the nucleic acid extraction process, and the final extraction results are also placed in open test tubes
Moreover, these extraction devices are bulky, costly, and require a large amount of consumables

Method used

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Embodiment Construction

[0033] Specific embodiments of the present invention are provided below, and it should be noted that the present invention is not limited to the following specific embodiments, and all equivalent transformations done on the basis of the technical solutions of the present application all fall within the scope of protection of the present invention.

[0034] Comply with the above technical solutions, such as Figure 1 to Figure 9 As shown, this embodiment provides a single-sample nucleic acid closed-type extraction instrument, including a cabin body 1 with a safety door 2, a magnetic bead moving mechanism 3 is installed on the inner top surface of the cabin body 1, and a magnetic bead moving mechanism 3 is installed on the inner bottom surface of the cabin body 1. There is a test tube group 5, and a placement tube 18 is processed on the test tube group 5, and a magnetic bar protective cover 19 is placed in the placement tube 18; the side wall of the cabin body 1 is provided with ...

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Abstract

The invention provides a single sample nucleic acid enclosed extractor. The single sample nucleic acid enclosed extractor comprises a cabin with safety door, the inner top surface of the cabin is provided with a magnetic bead moving mechanism, the inner bottom surface of the cabin is provided with a test tube group, an accommodating tube is processed on the test tube group, and a magnetic bar protective jacket is placed in the accommodating tube; the sidewall of the cabin is provided with a fan and a ventilation port for supplying air outside; the magnetic bar protective jacket is provided with a clamp hole; the inner top surface of the cabin is provided with a test tube cover moving mechanism, and a magnetic bar central axis in the magnetic bead moving mechanism, a test tube cover opening mechanical arm central axis in the test tube cover moving mechanism and a test tube central axis in the test tube group are in the same vertical plane. The single sample nucleic acid enclosed extractor avoids cross contamination among samples, the test tube is sequentially enclosed after each step, interference between test tubes is avoided, so that high-quality and high-purity extraction result can be obtained, and nucleic acid extraction efficiency is improved.

Description

technical field [0001] The invention belongs to the field of nucleic acid extraction, and relates to a nucleic acid extraction instrument, in particular to a single-sample nucleic acid closed extraction instrument. Background technique [0002] Acid extraction and purification is almost accompanied by the entire molecular biology research process, from the initial target gene extraction to PCR product purification or "gel recovery", as well as the extraction of plasmids in gene cloning and other experimental processes, are inseparable from nucleic acid extraction and purification, nucleic acid extraction Work is basically repetitive labor without any technical content. [0003] Nucleic acid extraction and purification is the starting point of molecular biology research, so nucleic acid extractors are widely used, including genomics research, such as extraction of DNA or RNA from animals, plants, microorganisms or viruses; molecular diagnosis of clinical samples, CDC; animal ...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12N15/1013
Inventor 李明苗保刚彭年才李政田春陈大山
Owner XIAN TIANLONG SCI & TECH
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