Butterfly orchid induction culture medium and asexual propagation method of butterfly orchid

An induction medium and asexual reproduction technology, applied in the field of plant tissue culture, can solve the problems of damaging the female parent, wasting internodes of pedicels, etc., and achieve the effects of fast growth, cost saving, and short induction time

Inactive Publication Date: 2014-05-07
TODAYS LANDSCAPING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the moth orchid tissue culture industrial production uses pedicel axillary buds to induce adventitious buds, and the internode part of the pedicel is discarded, which not only damages the female parent, but also wastes the internode part of the pedicel

Method used

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  • Butterfly orchid induction culture medium and asexual propagation method of butterfly orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of induction medium

[0033] (1) Prepare the basal mother solution of the Phalaenopsis induction medium according to the composition and concentration of the standard N6 medium, and divide it into 9 Erlenmeyer flasks, and label them respectively. No. 1 to No. 8 triangular flasks are the Phalaenopsis induction medium experimental group added with plant growth regulators and organic additives of the present invention, and No. 9 bottle is the medium control group only added with agar, white granulated sugar and activated carbon.

[0034] (2) According to Table 1, add 6-bean aminoadenine BA mother solution, tomato juice and activated carbon into each Erlenmeyer flask and mix with N6 basic mother solution to the desired concentration.

[0035] (3) Take a certain amount of tap water (replacing distilled water) and boil it. According to the concentration in Table 1, use an electronic balance to take agar and white sugar, pour the agar into boiling water ...

Embodiment 2

[0038] Embodiment 2: Inoculation and cultivation between pedicel internodes

[0039] Carry out inoculation in a sterile ultra-clean workbench, use a sterilized scalpel to cut off the incision where the two ends of the pedicel are in contact with the disinfectant, take the tender part between the two pedicel nodes, cut it into 2-3mm sections, and inoculate quickly Into each induction medium prepared in Example 1, seal with a sterilized bottle stopper after inoculation.

[0040] After inoculation, the culture bottle was placed in an artificial culture room, and the environmental parameters of the culture bottle were as follows:

[0041] Culture conditions: temperature 25±1°C, light intensity 1500-1800lx, light-dark alternation 12h / 12h.

[0042] After culturing for 50 days, the induction rate, induction time and growth of protocorms in each culture flask were recorded (see Table 1 for experimental data).

[0043] Table 1 Concentration of each component of induction medium and e...

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Abstract

The invention discloses a butterfly orchid induction culture medium and an asexual propagation method of butterfly orchid. The butterfly orchid induction culture medium is obtained by mixing a standard N6 culture medium serving as basic mother liquor with 6-benzylaminopurine BA, gibberellin GA3 and an organic additive. The butterfly orchid induction culture medium can be used for inducing pedicel internode parts of butterfly orchids to quickly propagate the butterfly orchids and has the advantages of low cost, high protocorm-like body induction rate, short induction time, high growth speed and the like. According to the asexual propagation method of the butterfly orchid, tender pedicel internodes are used as an explant, and the butterfly orchid induction culture medium is used as the culture medium; by the adoption of the method, pedicel materials are fully used; waste is turned into treasure, and the cost is reduced; furthermore, the parent is not damaged; the flowers on the lower parts of the pedicels can be still used for hybridization.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a phalaenopsis induction medium and a phalaenopsis asexual propagation method. Background technique [0002] Phalaenopsis B1.spp. is a tropical aerial orchid, which has large flowers, long flowering period, gorgeous flower color, rich color, beautiful and unique flower shape, dancing like a butterfly, hence the name. Known as the "Queen of Orchids" among tropical orchids, it is one of the most popular catayas in recent years. Phalaenopsis is a single-stem aerial orchid, and the plants rarely develop side buds, and the seeds are extremely difficult to germinate. Its conventional propagation is very slow, so tissue culture is often used for rapid propagation. At present, most of the Phalaenopsis tissue culture factory production uses pedicel axillary buds to induce adventitious buds, and discards the internodes of the pedicels, which not only damages the female parent, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002
Inventor 陈永得
Owner TODAYS LANDSCAPING BIOLOGICAL TECH
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