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Application of cyclic dipeptide C1 in Phellinus igniarius in resisting avian influenza H5N1 virus

A technology of Pyrenophora and cyclic dipeptide, applied in the directions of antiviral agents, applications, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2014-05-21
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A cyclic dipeptide has many important physiological activities such as antibacterial, so far, no report has been reported that has anti-influenza virus activity

Method used

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  • Application of cyclic dipeptide C1 in Phellinus igniarius in resisting avian influenza H5N1 virus
  • Application of cyclic dipeptide C1 in Phellinus igniarius in resisting avian influenza H5N1 virus

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] The crude extract of Pseudomonas firewood is obtained by the following method:

[0035] (1) The formula of the fermentation medium in grams / 100 ml is:

[0036] Corn Starch 1% Glucose 1%

[0037] Peptone 0.1% Yeast extract 0.1%

[0038] Magnesium sulfate 0.1% Potassium dihydrogen phosphate 0.01%

[0039] (2) by conventional method inoculated into the Erlenmeyer flask of liquid culture medium, with 25 ℃ of temperature, shake flask rotating speed is 110r / min, under pH 7 condition, shake culture for 7 days; When the pH value drops to 3, the seeds in the shake flask are inoculated into the culture solution of the 50L fermenter, and the temperature is 25°C, the pressure of the fermenter is 0.1 kg / cm 2 , pH 3, and the ventilation rate is 0.5-1.1vvm, and stirring Under the condition of a speed of 100 rev / min, and culturing for 7 days, the whole fermentation liquid of Mycelium of Pyrenospermum can be used to prepare the crude extract of Pyrenospermus.

[0040] (3) get the wh...

example 2

[0052] The crude extract of Pseudomonas firewood is obtained by the following method:

[0053] (1) The formula of the fermentation medium in grams / 100 ml is:

[0054] Cornstarch 3% Dextrose 2%

[0055] Peptone 0.5% Yeast extract 0.5%

[0056] Magnesium sulfate 0.5% Potassium dihydrogen phosphate 0.05%

[0057] (2) inoculate the Pyricularis sp. strain in the Erlenmeyer flask equipped with the liquid medium by a conventional method, at a temperature of 30 ° C, the rotating speed of the shake flask is 180 r / min, and under the condition of pH 6, the shaking culture is carried out for 15 days; When the pH value dropped to 2.5, the seeds in the shake flask were inoculated into the culture solution of the 50L fermentor, and the temperature was 30°C, the pressure of the fermenter was 0.2 kg / cm 2 , pH 3, the ventilation rate was 0.5-1.1vvm, and the stirring speed was The conditions of 180 rev / min, culturing for 15 days, the whole liquid fermentation of P. serrata mycelium can be use...

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Abstract

The invention discloses a cyclic dipeptide C1 with anti-avian influenza activity separated from Phellinus igniarius (LexFr) Quel (phellinus linteus (BerketCurt) Teng, Phellinus baumii, Phellinus hartigii (AlleschetSchnabl) Imaz) fermentation liquid and sporophores. The invention discloses new activity of cyclic dipeptide. The cyclic dipeptide is proved to have an anti-avian influenza action. Compared with the application of common compounds, the method detects the new activity of the cyclic dipeptide, and the cyclic dipeptide has the anti-avian influenza action.

Description

technical field [0001] The present invention relates to a method for extracting and preparing active substances from Phylonotus spp., which is resistant to avian influenza H5N1 virus, especially the S. General Microbiology Center of the Preservation Management Committee", the deposit number is CGMCC No.8108, the taxonomic name is Phellinus igniarius, and the deposit address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. Background technique [0002] Cyclic peptides usually referred to in biology refer to compounds formed by amino acid peptide bonds. In phytochemistry, this concept is expanded to a class of compounds formed by amide bonds, so the scope is expanded to include organic amines and macrocycles. Alkaloids are divided into cyclic peptides and linear peptides according to whether they form a ring or not. The reported cyclic peptide compounds have various biological activities, including anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4985A61P31/16C07D487/04A01G1/04
Inventor 赵晨宋爱荣孔超黄芳
Owner QINGDAO AGRI UNIV