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Construction method of pancreaticobiliary obstruction rat models

A technology of rat model and construction method, applied in medical science, veterinary surgery, surgery, etc., can solve problems such as inaccurate ligation, bile leakage, and low success rate

Inactive Publication Date: 2014-05-28
SHAOXING PEOPLES HOSPITAL
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Problems solved by technology

[0002] Acute severe cholangitis (acute cholangitis severe type, ACST) is a hot issue in the research of hepatobiliary surgery. Establishing a good animal model is the basis for experimental research on the disease. The most extensive ACST animal model is a rat model in which the biliary tract is completely obstructed and injected with bacteria or endotoxin. This type of model is irreversible and cannot simulate the clinical research after ACST relieves biliary obstruction.
There are also some literature reports that the pancreaticobiliary duct used in the pancreaticobiliary duct obstruction model is ligated at the opening of the intestinal duct, but the success rate of this method is not high, the ligation is not accurate, and it is difficult to establish models of cholangitis and pancreatitis; The way is easy to cause bile leakage and pancreatic leakage

Method used

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  • Construction method of pancreaticobiliary obstruction rat models
  • Construction method of pancreaticobiliary obstruction rat models
  • Construction method of pancreaticobiliary obstruction rat models

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Embodiment Construction

[0021] Materials and Methods

[0022] 1.1 General materials 26 clean and healthy Sprague-Dawley (SD) rats (provided by Zhejiang Experimental Animal Center) were selected, male, weighing 227-385 g, with an average (314.75±56.22) g. Standard granule mixed feed (provided by Zhejiang Experimental Animal Center) was fed, all rats were fasted during the experiment, and glucose 4.5 mg·kg was given intravenously -1 min -1 . The rats were paired and reared in pairs according to the age and weight of the rats, and were divided into two groups: ACST model group (ACST group, n =13) and sham operation group (SO group, n =13). There was no statistically significant difference in general data between the two groups ( P All > 0.05).

[0023] 1.2 Method

[0024] 1.2.1 Catheter preparation Choose a human epidural anesthesia catheter with an outer diameter of about 0.9 mm (produced by Zhejiang Haisheng Medical Instrument Co., Ltd.), and cut a section about 5 cm long from the proximal en...

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Abstract

The invention relates to a construction method of pancreaticobiliary obstruction rat models, and belongs to the technical field of animal pathological model construction. The method includes after an SD rat is narcotized, in a sterile environment, placing the segment of the pancreaticobiliary entering the distal part of the duodenum on a hard transparent support plate; slightly pressing the intestinal wall from opposite side edges of the mesenteric from the distal end of the opening of the pancreaticobiliary to allow digestive juice to accumulate in the segment of the duodenum where the pancreaticobiliary enters; using the same method, slightly pressing the duodenum at the proximal end of the opening of the pancreaticobiliary to allow the duodenum to fill through gastrointestinal pancreatic fullness; fixing the intestinal wall, inserting a closed venous indwelling needle into the intestinal wall in a non-vascular area on the opposite side of the opening of the pancreaticobiliary duct, inserting a puncture needle into the pancreaticobiliary duct, pulling out the needle core, inserting an indwelling hose, sealing the hose, and finishing a pancreaticobiliary obstructing model. According to the technical scheme, the construction method has the advantages of high success rate of puncture, high recoverability and the like.

Description

technical field [0001] The invention relates to a method for constructing a rat model of pancreaticobiliary obstruction, and belongs to the technical field of animal pathological model construction. Background technique [0002] Acute severe cholangitis (acute cholangitis severe type, ACST) is a hot issue in the research of hepatobiliary surgery. Establishing a good animal model is the basis for experimental research on the disease. Currently, the most widely used ACST animal model is complete biliary obstruction and injection of bacteria or internal medicine. Rat models of toxins are irreversible and cannot simulate clinical research after ACST relieves biliary obstruction. There are also some literature reports that the pancreaticobiliary duct used in the pancreaticobiliary duct obstruction model is ligated at the opening of the intestinal duct, but the success rate of this method is not high, the ligation is not accurate, and it is difficult to establish models of cholang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61B19/00
Inventor 鲁葆春
Owner SHAOXING PEOPLES HOSPITAL
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