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A fluorescein-labeled sialic acid reagent and its preparation method and application

A technology for fluorescein-labeled sialic acid, applied in the field of fluorescein-labeled sialic acid reagents and its preparation, to achieve good auxiliary effect, high specificity, and low background signal

Active Publication Date: 2016-03-09
厦门生光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To date, no literature has reported the application of sialic acid derivatives to track tumors [19]

Method used

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  • A fluorescein-labeled sialic acid reagent and its preparation method and application
  • A fluorescein-labeled sialic acid reagent and its preparation method and application
  • A fluorescein-labeled sialic acid reagent and its preparation method and application

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Embodiment Construction

[0049] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0050] 1. The intracellular distribution of the fluorescein-labeled sialic acid reagent provided by the present invention

[0051] Human cervical cancer cells (HeLa) were passaged from a 10cm cell dish to a special dish for cell confocal use, in 5% CO 2 , and cultivated in a cell culture incubator at 37°C for 24 hours. Suck off the old culture medium, pipette 1 μl of 0.1M FITC-NeuAc into 1 mL of DMEM medium with a pipette gun, suck off the old culture medium, add fresh medium containing nanoparticles and incubate for 1-24 hours, then Suck off the old culture medium, add the red fluorescent probe to the cell membrane, incubate for 10 min, wash with PBS three times, and observe the distribution of the fluorescein-labeled sialic acid reagent in the cells with a laser confocal fluorescence microscope. figure 1 It is the intracellular colocalization map ...

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Abstract

The invention relates to a fluorescein-labelled sialic acid reagent, a preparation method and an application, which belongs to the sialic acid reagent. A molecular formula of the fluorescein-labelled sialic acid reagent is C32H31N3O13S. According to the invention, N-N dimethyl formamide and potash are added in 9th position amino sialic acid and fluorescein isothiocyanate, after the reaction, the fluorescein-labelled sialic acid reagent is prepared; a molecular formula of the 9th position amino sialic acid is C11H20N2O8, and a molecular formula of fluorescein isothiocyanate is C21H11NO5S. The fluorescein-labelled sialic acid reagent can be used in preparation of a diagnosis agent before a tumour operation and a tracer in the tumour operarion. The specialty of the fluorescein-labelled sialic acid reagent is high, and the fluorescein-labelled sialic acid reagent specially enters into the tumour tissue, so that the fluorescein-labelled sialic acid reagent has low background signals, the fluorescein-labelled sialic acid reagent is successfully used in tumour small focuses so as to provide good auxiliary effect for tumor resection.

Description

technical field [0001] The invention relates to a sialic acid reagent, in particular to a fluorescein-labeled sialic acid reagent and a preparation method and application thereof. Background technique [0002] With the rapid development of the global economy, the incidence of cancer is increasing day by day. It is of great clinical significance to develop effective tumor tracing methods. Surgical resection has been widely used in the treatment of tumors, however, the ability to remove tumor lesions with the naked eye is limited, resulting in tumor recurrence. The development of fluorescent materials to aid in surgical resection could provide doctors with the exact location of tumors [1] . There is some clinical evidence for the application of fluorescence methods to tumor surgery, such as the use of fluorescein isothiocyanate-labeled folic acid to image epithelial ovarian cancer [4] . Conventional small molecule fluorescent dyes can be linked with tumor targeting ligands...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H7/027C07H1/00A61K49/00
Inventor 韩守法吴选俊田云鹏
Owner 厦门生光生物科技有限公司