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Preparation method of resistant starch RS3

A technology of resistant starch and starch, applied in the field of food biology, can solve the problems of unmentioned product yield, long cycle, low content of resistant starch, etc.

Inactive Publication Date: 2014-05-28
HENAN COOP MEDICAL SCI & TECH INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current Resistant Starch RS 3 The preparation methods include high-temperature autoclave method, acid method, enzymatic method (single-enzyme method or double-enzyme method) or a combination of multiple methods, but there are low resistant starch content (about 20%), long cycle (multiple freeze-thaw), And the product yield is not mentioned and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] (1) Mixing: use rice starch as raw material, prepare 14% starch milk, stir at 40°C for 1 hour; (2) Autoclave: adjust the pH value of starch milk to 4.0 with 2mol / L hydrochloric acid, add acidic α- Starch 0.24U / g dry starch, put it in a temperature-controllable internal heating high-pressure container, and gelatinize at 150°C for 30 minutes; (3) Debranching: Cool to about 55°C, and adjust the pH value to 5.0 with 2mol / L hydrochloric acid , add pullulanase 14U / g dry starch, and debranch at 55°C for 9 hours; (4) Centrifugation: Centrifuge the debranched liquid immediately, and centrifuge at 4000rpm for 15 minutes to obtain two parts, the heavy phase and the light phase; (5) Crystallization: Crystallize the heavy phase and the light phase at 5°C for 9 hours; (6) Sugar washing: wash the crystallized heavy phase and light phase with distilled water at 60°C for 2 times, and remove the supernatant by centrifugation to obtain the heavy phase and the light phase respectively. Pre...

Embodiment 2

[0011] (1) Mixing: use cornstarch as raw material, prepare 12% starch milk, stir at 60°C for 1 hour; (2) Autoclave: adjust the pH value of starch milk to 4.5 with 2mol / L hydrochloric acid, add acidic α- Starch 0.12U / g dry starch, put it in a temperature-controllable internal heating high-pressure container, and gelatinize at 155°C for 30 minutes; (3) Debranching: Cool to about 55°C, adjust the pH value to 4.5 with 2mol / L hydrochloric acid , add pullulanase 14U / g dry starch, and debranch at 55°C for 9 hours; (4) Centrifugation: Centrifuge the debranched liquid immediately, and centrifuge at 4000rpm for 20 minutes to obtain two parts, the heavy phase and the light phase; (5) Crystallization: Crystallize the heavy phase and the light phase at 5°C for 8 hours; (6) Sugar washing: wash the crystallized heavy phase and light phase with distilled water at 50°C for 2 times, and remove the supernatant by centrifugation to obtain the heavy phase and the light phase respectively. Precipit...

Embodiment 3

[0013] (1) Mixing: use potato starch as raw material, prepare 15% starch milk, stir at 55°C for 1 hour; (2) Autoclave: adjust the pH value of starch milk to 4.0 with 2mol / L hydrochloric acid, add acidic α- Starch 0.12U / g dry starch, put it in a temperature-controllable internal heating high-pressure container, and gelatinize at 160°C for 30 minutes; (3) Debranching: Cool to about 55°C, and adjust the pH value to 4.8 with 2mol / L hydrochloric acid , add pullulanase 12U / g dry starch, and debranch at 55°C for 8 hours; (4) Centrifugation: Centrifuge the debranched liquid immediately, and centrifuge at 4000rpm for 20 minutes to obtain two parts, the heavy phase and the light phase; (5) Crystallization: Crystallize the heavy phase and the light phase at 5°C for 9 hours; (6) Sugar washing: wash the crystallized heavy phase and light phase with distilled water at 50°C for 3 times, and remove the supernatant by centrifugation to obtain the heavy phase and the light phase respectively. P...

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Abstract

The invention provides a preparation method of resistant starch RS3. The preparation method of the resistant starch RS3 comprises the following steps: (1) pulping: preparing starch milk from starch (corn, wheat, rice, sweet potato and potato) as raw materials and pulping uniformly; (2) pressing and heating: adjusting the pH value of the starch milk by using hydrochloric acid, adding a certain amount of acidic alpha-amylase, placing in a temperature-controllable heating high-pressure container, and pressing and heating; (3) debranching: adding pullulanase into starch paste which is cooled after pressing and heating and debranching; (4) centrifuging: centrifuging the debranching liquid at once to obtain a heavy phase part and a light phase part; (5) crystallizing: crystallizing the heavy phase part and the light phase part respectively at low temperature; (6) washing sugar: performing sugar washing on the crystallized heavy phase and the crystallized light phase respectively by using distilled water with a certain temperature for many times, centrifuging and removing supernatant to obtain heavy phase precipitate and light phase precipitate respectively; (7) performing fluidized drying by using a fluidized bed: drying and crushing the heavy phase precipitate and the light phase precipitate respectively to obtain two different specifications of resistant starch RS3.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a dual-enzyme synergistic, phase-separated crystallization preparation method of resistant starch. Background technique [0002] At present, some grain starches in our country have excess demand and low added value, so it is urgent to further process them to obtain modified starches with different functional types. where RS 3 Type resistant starch is an emerging field of food research in the world in recent years. It can reduce insulin response, relieve type 2 diabetes, regulate body energy intake, and control body weight, so it has broad prospects in human health diet. The book "Carbohydrates in Human Nutrition" jointly published by the World Health Organization and other institutions in 1998 pointed out: "The discovery and research progress of resistant starch is an important achievement in the research on the relationship between carbohydrates and health in rece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B30/00C08B30/20C08J3/00
Inventor 苏雪锋冯军伟杨铭乾王文游倩倩
Owner HENAN COOP MEDICAL SCI & TECH INST CO LTD
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