Method for determining fluorescence spectrum of hydrophobic association polymer

Abstract

The invention discloses a method for determining the fluorescence spectrum of a hydrophobic association polymer. The method comprises the following steps: 1, preparing a hydrophobic associating polymer sample for later use; 2, preparing a methanol solution of pyrene; 3, sucking 50.0muL of the methanol solution of pyrene by a microsyringe, transferring the methanol solution of pyrene to a 50mL clean volumetric flask, and introducing nitrogen under shaking to blow away methanol, dry pyrene and spread pyrene on the wall of the volumetric flask; 4, weighing a proper amount of the hydrophobic associating polymer, putting the hydrophobic associating polymer in the volumetric flask, adding a quantitative amount of distilled water to prepare different concentrations of a polymer solution, putting the obtained polymer solutions in constant temperature water-bath oscillators to completely disperse and dissolve pyrene; and 5, introducing nitrogen to remove oxygen in the solutions, allowing the obtained solutions to stand at normal temperature, and carrying out fluorescence spectroscopy of the hydrophobic associating polymer solutions. The method can be successfully used to determine the fluorescence spectrum of the hydrophobic association polymer, and has the advantages of accurate test result, simple test steps, and substantial reduction of the test cost.

Description

technical field [0001] The invention relates to a method for measuring the fluorescence spectrum of hydrophobically associated polymers. Background technique [0002] Hydrophobic association polymer refers to a water-soluble polymer with a small amount of hydrophobic groups on the hydrophilic macromolecular chain of the polymer. A small amount of hydrophobic groups are introduced into the water-soluble macromolecular chain to make its aqueous solution exhibit unique rheological properties. Above a certain polymer concentration, the hydrophobic parts associate to form a dynamic three-dimensional network structure, thereby forming a large supramolecular chain aggregate, increasing the hydrodynamic volume of the polymer, and significantly increasing the viscosity of the solution. The addition of small molecule electrolytes can increase the polarity of the solvent, enhance the hydrophobic association, and produce obvious calcium chloride resistance. Under the action of high sh...

AI Technical Summary

Problems solved by technology

Techniques for probing the properties of macroscopic solutions, such as viscosity determination and hydrodynamic studies, have certain guiding significance for the study of molecular aggregation behavior of hydrophobically associated water-soluble polymers, but there are almost no analytical methods for probing intermolecular interactions at the molecular level