Application of FOXE1 gene and WNT3 gene as well as methylation amplimer and probe of FOXE1 gene and WNT3 gene

An amplification primer and methylation technology, applied in the field of methylation amplification primers and probes, can solve the problem of not finding tumor markers, etc.

Inactive Publication Date: 2014-06-11
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Tumor markers have the advantages of minimally invasive, convenient detection, low cost, and convenient general screening, etc., and have high sensitivity and specificity. The occurrence and development of colorectal cancer are the result of multiple gene damage, and no ideal tumor has been found yet. landmark

Method used

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  • Application of FOXE1 gene and WNT3 gene as well as methylation amplimer and probe of FOXE1 gene and WNT3 gene
  • Application of FOXE1 gene and WNT3 gene as well as methylation amplimer and probe of FOXE1 gene and WNT3 gene
  • Application of FOXE1 gene and WNT3 gene as well as methylation amplimer and probe of FOXE1 gene and WNT3 gene

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Embodiment

[0017] 1. Materials and methods:

[0018] 1. Materials: normal colorectal mucosa (approximately 50 cases) were obtained from colonoscopic biopsy specimens, colorectal cancer tissues and adjacent normal tissues (approximately 300 cases) were obtained from surgical resection specimens, and all cases were newly diagnosed patients. Whole blood, serum and plasma from patients with colorectal cancer (312 cases) and normal controls (200 cases) were collected without radiotherapy and chemotherapy and confirmed by pathology. Patients with colorectal cancer included 181 males and 131 females. Aged 38 - 82 years old, with an average of 61.6 ± 12.3 years old. 200 healthy subjects were selected as the normal control group, including 118 males and 82 females. Aged 21-71 years old, average age 40.21 ± 18.05 years old. Specimens were obtained with the informed consent of the patients and approved by the Medical Ethics Committee of Run Run Shaw Hospital.

[0019] 2. Method:

[0020] 2.1 D...

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Abstract

The invention discloses an application of FOXE1 gene and WNT3 gene as well as a methylation amplimer and a probe of the FOXE1 gene and the WNT3 gene, belonging to the field of biotechnology. The invention provides an application of the FOXE1 gene and / or WNT3 gene as a methylation colorectal cancer tumor marker and a methylation amplimer and a probe of the FOXE1 gene and WNT3 gene. According to the invention, the FOXE1 gene and WNT3 gene can be used as a tumor marker in colorectal cancer, the sensitivity by combining the two methylation tumor markers can reach 79%, the specificity is 59%, and even the sensitivity of the I-stage tumor patient can reach 63.3%, which indicates the feasibility of finding a methylation tumor marker in whole-blood DNA. The methylation amplimer and the probe disclosed by the invention can detect the obvious difference between the FOXE1 gene and the WNT3 gene in the methylation state in a colorectal cancer patient and normal control whole-blood DNA.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of FOXE1 gene and WNT3 gene and the methylation amplification primer and probe of the gene. Background technique [0002] Colorectal cancer is one of the common malignant tumors in my country, and its high morbidity and mortality have posed a serious threat to human health. At present, colonoscopy has the highest sensitivity and specificity for the detection of colorectal cancer, but due to reasons such as high cost, greater pain for patients and high operation requirements, the willingness of patients to do this test has been reduced, resulting in many patients being delayed to an advanced stage. Tumor markers have the advantages of minimally invasive, convenient detection, low cost, and convenient general screening, etc., and have high sensitivity and specificity. The occurrence and development of colorectal cancer are the result of multiple gene damage,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 胡晓彤何超
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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