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Cold inducible expression promoter Poscold3 of plant stem leaf and application of promoter

A technology of plant stems and leaves, cold induction, applied in the field of biotechnology and plant genetic engineering, can solve the problems of many unfavorable traits and difficult improvement, and achieve the effect of increasing expression and high biological safety

Inactive Publication Date: 2014-06-18
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, local cold-tolerant rice resources often have many unfavorable traits, making it extremely difficult to improve

Method used

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  • Cold inducible expression promoter Poscold3 of plant stem leaf and application of promoter
  • Cold inducible expression promoter Poscold3 of plant stem leaf and application of promoter
  • Cold inducible expression promoter Poscold3 of plant stem leaf and application of promoter

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Embodiment Construction

[0029] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0030] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0031]Acquisition of Poscold2 Promoter Containing Restriction Sites

[0032] Step 1. Design of primers

[0033] According to the whole genome sequence of the rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice PCole1 gene, and the restriction sites of the primers were designed according to the characteristics of the selected vector and target gene.

[003...

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Abstract

The invention provides a cold inducible expression promoter Poscold3 of a plant stem leaf and an application of the promoter. The invention further provides an expression box with the promoter, a plant expression vector, a host bacterium and a converter. Particularly, the invention obtains the promoter and uses the promoter in plant transgenetic engineering. The promoter provided by the invention can drive an exogenous gene to be specifically expressed in stems and leaves of a plant under a cold inducible condition. The promoter is suitable for monocotyledon cereal, and particularly can drive the exogenous gene to be expressed under induction in stems and leaves of rice plant, so that the promoter can be used for enhancing and improving the growth characteristic and cold resistance of rice, thereby cultivating an ideal rice variety.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant stem and leaf cold-inducible gene expression promoter and its application. The promoter can drive the expression of a target gene in a plant under cold-induced conditions in a plant transgenic regulation system. Background technique [0002] Rice is an important food crop in my country, and chilling injury is one of the important limiting factors affecting rice production. The impact of low temperature chilling injury on rice yield is mainly manifested in the reduction of seed setting rate, delayed pollen development, poor anther dehiscence, affecting fertilization and setting, resulting in a decrease in yield. Rice breeders are eager to transfer the strong cold tolerance of local resources to cultivated varieties through breeding methods, so as to improve cold tolerance and reduce rice yield loss caused ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/84C12N1/21A01H5/00C12R1/01
Inventor 魏鹏程李莉杨剑波张银萍李浩秦瑞英马卉倪大虎杨亚春宋丰顺
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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