Method for measuring lignin content of herbaceous plants

Abstract

The invention relates to a method for measuring the lignin content of herbaceous plants. The method comprises the following steps: (1) drying, smashing and sieving a plant sample to obtain plant sample powder; (2) adding acetic acid to the plant sample powder to obtained fully-softened plant sample powder; (3) carrying out centrifugation to obtain a precipitate A; using the acetic acid to clean the precipitate A; (4) embathing the precipitate A into an ethanol-ether solution to obtain a precipitate B; (5) after the precipitate B is dried by distillation, adding sulfuric acid for stirring to be uniform, and then standing at room temperature; (6) adding distilled water for stirring to be uniform; after boiling water bath and cooling at the room temperature, sequentially adding the distilled water and barium chloride to shake up for centrifugation to obtain a precipitate C; (7) after the precipitate C is flushed with water, sequentially adding the sulfuric acid and potassium dichromate, and cooling to the room temperature after reaction; (8) transferring a substance obtained in the step (7) into a conical flask; (9) after adding potassium iodide into the conical flask for reaction, adding a starch indicator, using a sodium thiosulfate solution to titrate to a terminal point; (10) carrying out a blank test; (11) calculating the lignin content. The method is simple, convenient and accurate.

Description

technical field [0001] The invention relates to a measuring method, in particular to a measuring method for the lignin content of herbal plants. Background technique [0002] As we all know, lignin is the second organic polymer compound after cellulose in nature. Lignin is mainly distributed in the tubular molecules and fibers of the xylem in plants, sclerenchyma cells, sclerenchyma cells, and the secondary walls of specific types of epidermal cells, and constitutes the three main components of plant skeletons together with cellulose and hemicellulose. The body mainly plays the functions of mechanical support, preventing biodegradation, disease defense, and transporting water. Lignin is formed by the polymerization of three types of phenylpropenyl alcohols (single woody alcohols): coumarly alcohol, coniferly alcohol and sinapya alcohol. Because lignin monomers are different, lignin can be divided into three types: syringyl lignin (S-lignin) polymerized from syringyl propan...

AI Technical Summary

Problems solved by technology

This method simplifies the process of processing plant materials, resulting in large errors in the determination of lignin content.

In addition, except for the above-mentioned several invention patents related to the method of measuring lignin in plants, there are few or no patents on other similar methods of measuring lignin, which shows that there are still many problems in the method of lignin determination that need to be further studied

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