Phase microscope imaging method based on SHWS (Shack-Hartmann Wavefront Sensor)
A phase microscope and imaging method technology, applied in the field of microscope imaging, can solve the problems of cumbersome reconstruction process, vibration noise, complex hardware structure and so on
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[0029] In order to describe the present invention more specifically, the technical solutions of the present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0030] Such as figure 1 Shown, a phase microscope imaging method based on SHWS, comprises the following steps:
[0031] (1) For the two cases of not putting the sample and putting the sample, use SHWS to detect and obtain the Hartmanngram corresponding to the light at each angle in the two cases;
[0032] Such as figure 2As shown, the helium-neon laser beam He-Ne (λ=633nm) is first incident on the scanning galvanometer GM. Then, after passing through lens L1 (f=200mm) and oil-immersed condenser lens CL (Nikon, NA=1.4) in turn, it is irradiated onto the sample. A 4f optical system is formed between the GM and the sample. The sample is immersed in a solution with a certain refractive index and placed between two coverslips, which are separated and seal...
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