Extraction method of Symphytum officinale leaf protein

An extraction method and a technology of aggregated grass leaves, which are applied in the agricultural field, can solve the problems that the extraction method of aggregated grass leaf protein has not been discovered, and achieve the effect of simple and feasible process

Inactive Publication Date: 2014-07-02
李宝霞
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  • Abstract
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  • Claims
  • Application Information

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In the prior art, no relevant reports have been found abo

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  • Extraction method of Symphytum officinale leaf protein
  • Extraction method of Symphytum officinale leaf protein
  • Extraction method of Symphytum officinale leaf protein

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Embodiment Construction

[0022] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0023] Materials and Methods

[0024] Material

[0025] Polygrass was collected from Shanxi Agricultural University Family Academy. The fresh grass was washed, dried in a constant temperature oven at 60°C, and then pulverized.

[0026] Main instruments and equipment

[0027] Acidity meter (PHS-3C), Shanghai Hongyi Instrument Co., Ltd.; vortex oscillator, Shanghai Jingke Industrial Co., Ltd.; refrigerator (BCD-183), Chongqing Wuzhou Industrial Company, China; desktop high-speed refrigerated centrifuge, Shanghai Lizhong Science Instrument Co., Ltd.; digital constant temperature water bath (HH-4), Jintan City Kexi Instrument Co., Ltd.; visible scenery photometer (WFJ2100), Longnic (Shanghai) Instrument Co., Ltd.; electronic balance (BS224S), Beijing Sai Doris Instrument System Co., Ltd.; and Kjeldahl nitrogen...

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Abstract

The invention discloses an extraction method of Symphytum officinale leaf protein, which comprises the following steps: dissolving protein in a dilute base solution, and separating out insoluble high-polymer components; regulating to isoelectric point with acid to precipitate the protein, removing the non-acid-precipitation soluble components, washing, regulating the pH value to a neutral state, homogenizing, and carrying out freeze-drying and the like to prepare the higher-purity protein. The result proves that the method has favorable effect, and the protein extraction rate is up to 63.42. The optimum extraction conditions are as follows: the concentration of the NaOH solution is 0.15 mol/L, the solid-to-liquid ratio is 1:40, the extraction time is 100 minutes, the extraction temperature is 60 DEG C, and the extraction rate is up to 63.42%.When the pH value is 2.55-3.51, the crude protein precipitation effect in the Symphytum officinale is optimal.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to a method for extracting aggregated grass leaf protein. Background technique [0002] Leaf protein is a protein product directly extracted from the fresh stems and leaves of green plants. It is a non-toxic and harmless natural high-grade nutritional product, and the protein content of its products is between 50% and 80%. Leaf protein is rich in 18 kinds of amino acids, among which the content of 8 kinds of essential amino acids is high. It is an excellent protein feed. The results of a large number of animal feeding experiments at home and abroad show that leaf protein can completely replace soybean meal and fish meal. In addition, the content of lutein in leaf protein products is as high as 1000mg / kg-1300mg / kg. Lutein is a natural coloring agent for poultry, meat, skin and egg yolk, which is of great significance in the production of poultry industry. In the international mark...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/30
CPCC07K1/145C07K1/30C07K1/36
Inventor 李宝霞霍乃蕊董双涛宋金玉杜月连刘精婵贾文雅
Owner 李宝霞
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