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Quantitative determination method for organophosphorus pesticides in fresh tea leaves

A quantitative detection method, organophosphorus pesticide technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of insufficient stability and reliability of detection results, high consumption of organic solvents, cumbersome operations, etc., and achieve stable results, low detection limits, good repeatability

Active Publication Date: 2014-07-09
江苏太湖地区农业科学研究所
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Problems solved by technology

[0006] The invention provides a quantitative detection method for organophosphorus pesticides in fresh tea leaves, the purpose of which is to solve the problem of long time-consuming, cumbersome operation, high consumption of organic solvents and insufficient stability of detection results of existing detection methods for the determination of organophosphorus pesticides in tea leaves reliable question

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  • Quantitative determination method for organophosphorus pesticides in fresh tea leaves
  • Quantitative determination method for organophosphorus pesticides in fresh tea leaves
  • Quantitative determination method for organophosphorus pesticides in fresh tea leaves

Examples

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Embodiment

[0040] Example: A Quantitative Detection Method of Organophosphorus Pesticides in Fresh Tea Leaves

[0041] The fresh leaves of the tested tea were taken from a local tea plantation in Suzhou, crushed and mixed by a pulverizer, put into a clean container, and stored in a -20°C refrigerator for later use. The quantitative detection method consists of two parts:

[0042] The first part, establishes the standard curve of the measured organophosphorus pesticide of known gradient concentration with gas chromatography; The establishment of described standard curve is made up of the following steps:

[0043] The first step is to prepare a blank matrix extract, and the preparation method consists of the following steps:

[0044] (1) Weigh 5.0g of blank tea leaf samples that are determined to be free of the tested organophosphorus pesticides and pyrethroid pesticides, place them in a 50mL centrifuge tube, and then add 10mL of acetic acid containing 1% volume fraction to the centrifuge...

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Abstract

The invention provides a quantitative determination method for organophosphorus pesticides in fresh tea leaves. The quantitative determination method is characterized by comprising the following steps: carrying out ultrasonic extraction on fresh tea leaf test samples by using an acetonitrile solution containing 1% of acetic acid by volume percent; purifying by using a suitable amount of PSA (Primary Secondary Amine), C18 and GCB (Graphitized Carbon Black) mixed fillers; blowing liquid supernatant by nitrogen and concentrating until the liquid supernatant is nearly dried; dissolving by acetone and enabling the volume to be constant; raising the temperature by using a DB-1701 quartz capillary column and separating; carrying out GC / FPD (Gas Chromatograph / Flame Photometric Detector) detection; and finally, quantifying by using a base material external standard method. A test confirms that the method has a wide linear range; the technical indexes including detection sensitivity, accuracy and precision meet the requirements of a residue analysis; the pre-treatment operation is simple, convenient and rapid and a reliable manner is provided for analytical researches for determining the organophosphorus pesticides in fresh tea leaves.

Description

technical field [0001] The invention relates to the field of detection methods for pesticide residues, in particular to a quantitative detection method for organophosphorus pesticides in fresh tea leaves. Chromatography is used to detect organophosphorus pesticides in order to achieve the purpose of fast, simple and reliable. Background technique [0002] Tea is one of the three major plant beverages in the world. It is more and more popular because of its unique taste and health effects such as anti-cancer, anti-mutation, scavenging free radicals, and delaying aging. my country is a big tea producing country and also a big exporting country. The application of pesticides is the most effective means to ensure the yield of tea and control the threat of pests and diseases during its growth. Organophosphorus pesticides (English name: Organophosphorus pesticides) are widely used due to their broad spectrum, high efficiency and low cost. , less dosage is widely used in the preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 张丽陆皓茜董明辉杨代凤邓金花顾俊荣刘腾飞钱辉
Owner 江苏太湖地区农业科学研究所
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