Method for improving yield of baicalin from hairy roots of scutellaria baicalensis

A technology for hairy root and glycoside yield, which is applied in the field of plant tissue culture to achieve the effects of increasing baicalin content and simple operation

Inactive Publication Date: 2014-07-16
西藏欧塞曼洛医药科技有限公司
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  • Method for improving yield of baicalin from hairy roots of scutellaria baicalensis
  • Method for improving yield of baicalin from hairy roots of scutellaria baicalensis
  • Method for improving yield of baicalin from hairy roots of scutellaria baicalensis

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[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0024] The present invention utilizes low-energy N + Inject fresh Scutellaria baicalensis hairy roots and use them as seeds for three-stage Scutellaria baicalensis hairy root cultivation. The specific steps are as follows:

[0025] 1) Pre-cultivation: Inoculate the hairy roots of Scutellaria baicalensis Georgi (acquisition method according to ZL201110132376.4) in 100 mL of modified MS under sterile conditions with an inoculum of 5 g / L. in liquid medium, and then cultured in the dark at 26°C for 6 days, and the improved MS liquid medium was in MS liquid medium (Murashige T and Skoog F(1962).Physiol Plant15(3):473-497.) Add 0.4mmol / L phenylalanine and 60g / L sucrose;

[0026] 2) After step 1), weigh 150 mg of the above-mentioned pre-cultured hairy roots on the aseptic operating table and cut them into 1.5 cm hairy root fragments, and place the hairy ro...

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Abstract

The invention provides a method for improving the yield of baicalin from hairy roots of scutellaria baicalensis. The method mainly comprises the following steps: processing the hairy roots of scutellaria baicalensis by using a low-energy ion beam injection technology, with the processed hairy roots of scutellaria baicalensis as seeds, performing enlarge culturing by using a three-stage culture method, and measuring baicalin content by high efficiency liquid chromatography; the result shows that, compared with a control group, the hairy roots which are subjected to induced mutation by the low-energy ions have the advantages of high growing speed, high capability of biosynthesizing baicalin, genetic stability and the like, when the hairy roots of scutellaria baicalensis grow for 45 days, the biomass and the baicalin content of the hairy roots respectively are 16.57g/L and 28.3g/L, which are improved by 24.77% and 35.03% respectively than those of the hairy roots, respectively being 13.28g/L and 21.01%, which are not injected with the low-energy ions.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for increasing the yield of baicalin in hairy roots of Scutellaria baicalensis. Background technique [0002] Scutellaria baicalensis is a commonly used medicinal material, which has the functions of clearing heat and dampness, purging fire and detoxification, stopping bleeding, and preventing miscarriage. Its main active ingredients, baicalin and baicalein, have significant antipyretic, antibacterial, sedative, and hypotensive effects. Therefore, manufacturers of extracting active ingredients of traditional Chinese medicines using Scutellaria baicalensis as raw materials have emerged one after another. It is the Shuanghuanglian and Yinhuang series, and the annual sales of Scutellaria baicalensis have increased sharply. In addition, the country has issued a series of policies to protect wild resources in recent years, resulting in the dwindling ...

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Application Information

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IPC IPC(8): A01H1/06A01H4/00C07H17/07
Inventor 钱卫东付云芳施春阳蔡长龙齐香君
Owner 西藏欧塞曼洛医药科技有限公司
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