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Detection method of Prunella vulgaris fingerprint

A fingerprint and detection method technology, which is applied in the field of medicinal material detection, can solve the problems of few detection methods, complexity, and large error in results, and achieve the effects of saving reagents, high precision, and accurate results

Active Publication Date: 2015-12-30
GUANGZHOU WANGLAOJI MAJOR HEALTH IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are some Prunella vulgaris powders that have lost the original form of Prunella vulgaris and a wide variety of compound medicines containing Prunella vulgaris in the market, and the methods for detecting whether these substances are Prunella vulgaris or whether they contain Prunella vulgaris are relatively complicated in the prior art. And the result error is large; in addition, although Prunella vulgaris can be used as a whole plant, the medicinal effect of the fruit ear is better than other parts, but there are relatively few detection methods for distinguishing the fruit ear from other parts

Method used

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  • Detection method of Prunella vulgaris fingerprint
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  • Detection method of Prunella vulgaris fingerprint

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A detection method of Prunella vulgaris fingerprint, comprising the following steps:

[0025] (1) Preparation of the test solution: Pulverize Prunella vulgaris through a 60-mesh sieve, accurately weigh 0.5g, place it in a 100mL conical flask, add methanol aqueous solution with a volume fraction of 75%, and dissolve it by ultrasonic (power 250W, frequency 33kHz ), 30min, let cool, set the volume to the mark, shake well, filter, take the filtrate as the test solution;

[0026] (2) Preparation of the reference substance solution: accurately weigh the rosmarinic acid reference substance, add a methanol solution with a volume fraction of 75% to dissolve, and make a solution containing 15 μg of rosmarinic acid in every 1 mL, as the reference substance solution;

[0027] (3) Accurately draw need testing solution and reference substance solution respectively, inject in the ultra-high performance liquid chromatograph, measure, get the fingerprint collection of Prunella vulgaris,...

Embodiment 2

[0030] A detection method of Prunella vulgaris fingerprint spectrum, is characterized in that, comprises the following steps:

[0031] (1) Preparation of the test solution: crush Prunella vulgaris through a 55-mesh sieve, accurately weigh 1g, place it in a 100mL Erlenmeyer flask, add 60% methanol aqueous solution, and ultrasonically dissolve (power 240W, frequency 30kHz) , 25min, let cool, constant volume, shake well, filter, take the filtrate as the test solution;

[0032] (2) Preparation of the reference substance solution: accurately weigh the rosmarinic acid reference substance, add a volume fraction of 60% methanol solution to dissolve, and make a solution containing 14 μg of rosmarinic acid in every 1 mL, as the reference substance solution;

[0033] (3) Accurately draw the test solution and the reference solution respectively, inject them into an ultra-high performance liquid chromatograph, measure, and obtain the fingerprint of Prunella vulgaris (its 15 common peaks, a...

Embodiment 3

[0036] A detection method of Prunella vulgaris fingerprint spectrum, is characterized in that, comprises the following steps:

[0037] (1) Preparation of the test solution: crush Prunella vulgaris through a 65-mesh sieve, accurately weigh 2g, place it in a 10mL Erlenmeyer flask, add methanol, dissolve it by ultrasonic (power 240W, frequency 30kHz), 35min, let cool, and set volume, shake well, filter, and take the filtrate as the test solution;

[0038] (2) Preparation of the reference substance solution: accurately weigh the rosmarinic acid reference substance, add methanol to dissolve, and make a solution containing 16 μg of rosmarinic acid in every 1 mL, as the reference substance solution;

[0039] (3) Accurately draw the test solution and the reference solution respectively, inject them into an ultra-high performance liquid chromatograph, measure, and obtain the fingerprint of Prunella vulgaris (its 15 common peaks, and the No. 7 peak is identified as rosmarinic acid) ; ...

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Abstract

The invention discloses a detection method for a prunella vulgaris fingerprint spectrum. The detection is carried out by adopting ultra-high performance liquid chromatography. The method comprises the following steps: preparing a test solution, namely crushing prunella vulgaris, precisely weighing, adding a 60-100% methanol solution, carrying out ultrasound dissolving, naturally cooling, making to constant volume, shaking uniformly, and filtering to obtain filter liquor which is used as the test solution; preparing a comparison product solution, namely precisely weighing a rosmarinic acid comparison product, and adding the 60-100% methanol solution for dissolving so as to obtain the comparison product solution; and respectively precisely absorbing the test solution and the comparison product solution, and injecting the test solution and the comparison product solution into a ultra-high performance liquid chromatograph for measuring so as to obtain the prunella vulgaris fingerprint spectrum comprising 15 peaks. The detection method for the prunella vulgaris fingerprint spectrum can be obtained by carrying out a large number of tests and researches by the inventor, the test solution and the comparison product solution are prepared, and optimal parameters of chromatographic conditions of the ultra-high performance liquid chromatograph can be determined; by using the method, the results are precise, the precision is high and reagents are saved.

Description

technical field [0001] The invention relates to the field of detection of medicinal materials, in particular to a detection method of the fingerprint of Prunella vulgaris. Background technique [0002] Prunella vulgaris is a plant of the Labiatae family of the order Lamiformes. It is cold in nature, sweet, pungent, and slightly bitter. It has the effects of clearing liver fire, dispelling stagnation and swelling, clearing heat and detoxification, eliminating phlegm and relieving cough, cooling blood and stopping bleeding. It is suitable for lymphatic tuberculosis , goiter, mastitis, dizziness, crooked mouth and eyes, muscle and bone pain, tuberculosis, metrorrhagia, leukorrhagia, acute infectious jaundice hepatitis and bacillary dysentery, etc. Modern pharmacological studies have shown that Prunella vulgaris also has the effect of lowering blood pressure. [0003] At present, there are some Prunella vulgaris powders that have lost the original form of Prunella vulgaris and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 蒋莉娟姚江雄许招懂方铁铮林丽美黎志坚
Owner GUANGZHOU WANGLAOJI MAJOR HEALTH IND
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