A kind of tissue culture rapid propagation medium and tissue culture rapid propagation method of dendrobium

Abstract

The invention discloses a dendrobium flexicaule tissue culture and rapid propagation culture medium as well as a tissue culture and rapid propagation method. The culture medium consists of magnesium sulfate heptahydrate, monopotassium phosphate, ammonium sulfate, calcium nitrate terahydrate, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, molybdenum trioxide, ferrous sulfate heptahydrate, ethylenediaminetetraacetic acid disodium salt, 6-benzylaminopurine, 1-naphthylacetic acid, agar, sugarcane, white sugar and banana sauce; the pH value of the culture medium is 5.6-5.8; the tissue culture and rapid propagation method comprises steps of sterilization of the culture medium, seed germination and culture, proliferation, rooting culture, seedling hardening and transplanting, and is simple, convenient and easy to implement according to the characteristics of dendrobium flexicaule; the tissue-cultured seedling clusters are strong, free of malformation and deep green in leaf color; the propagation coefficient is increased; the propagation period is about 50 days; the culture effect is good; the survival rate is high.

Description

technical field [0001] The invention belongs to the field of tissue culture rapid propagation methods of plants, in particular to a tissue culture rapid propagation medium and a tissue culture rapid propagation method of Dendrobium bentuli. Background technique [0002] Dendrobium dendrobii, cylindrical, slightly curved, 2 to 4 leaves, alternate on the upper part of the stem, nearly leathery, oblong-lanceolate, about 3 cm long, inflorescences from the upper part of the old stem that has fallen leaves , with 1 to 2 flowers, bracts light white, ovate-triangular, about 3 mm long, apex acute; pedicel and ovary yellow-green with lavender, flowers spread out, middle sepals back yellow-green, upper end slightly lavender, Oblong, the lower part of the petals is yellow-green, the upper part is nearly lavender, oval, about 25 mm long, 13 mm wide in the middle, blunt at the apex, with 5 veins, flowering in May, produced in Henan (Funiu Mountains), Hubei (Shennongjia) region), eastern ...

AI Technical Summary

Problems solved by technology

In terms of planting methods, the local herb farmers continue to use the traditional method of digging in the field and planting by division, which consumes a large amount of wild resources of Dendrobium candidum, resulting in scarcity of germplasm resources, low reproduction coefficient, slow development speed, and inability to effectively expand the planting scale.

Moreover, due to the backward cultivation technology and extensive and simple management, the survival rate of Dendrobium candidum is low, the growth cycle is long, the yield is low, and the benefit is poor.