Method for rapid preparation of sodium hyaluronate from sodium hyaluronate fermentation broth
A technology of sodium hyaluronate and fermentation liquid, which is applied in the field of preparation of sodium hyaluronate, can solve the problems of low protein content, low cost, and high purity of powdered HA, reduce the yield of HA, reduce production time, and save complex The effect of melting time
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Embodiment 1
[0014] 1) Take 1000ml of fermented broth, containing 4g of HA, with a molecular weight of 2.26MDa.
[0015] 2) The temperature of the fermentation broth is raised to 100° C., maintained for 20 minutes, and filtered to obtain a filtrate.
[0016] 3) The filtrate was diluted to 3000 ml with purified water, 20 g / L of activated carbon and 2 g / L of diatomaceous earth were added, stirred and adsorbed for 4 hr, and filtered to obtain the filtrate.
[0017] 4) Add 500 g of D113 resin to the filtrate, stir and absorb for 1 hour, then filter off the resin to obtain the filtrate.
[0018] 5) Add sodium chloride to the filtrate to make the final concentration 0.4mol / L, use 2-3 times of ethanol to precipitate HA, then wash, solidify and dry to obtain powdered HA.
[0019] The whole process took 8 hours, and the obtained HA dry powder was 3.8g, the purity of glucuronic acid was about 43.6%, the molecular weight was 1.67MDa, protein / HA (g / g)=0.08%.
Embodiment 2
[0021] 1) Take 1000ml of fermented broth, containing 4g of HA, with a molecular weight of 2.26MDa.
[0022] 2) The temperature of the fermentation broth was raised to 121° C., maintained for 10 minutes, and filtered to obtain a filtrate.
[0023] 3) The filtrate was diluted to 4000 ml with purified water, 50 g / L of activated carbon and 2 g / L of diatomaceous earth were added, stirred and adsorbed for 2 hr, and filtered to obtain the filtrate.
[0024] 4) Add 500 g of D113 resin to the filtrate, stir and absorb for 2 hours, then filter off the resin to obtain the filtrate.
[0025] 5) Add sodium chloride to the filtrate to make the final concentration 0.4mol / L, use 2-3 times of ethanol to precipitate HA, then wash, solidify and dry to obtain powdered HA.
[0026] The whole process takes 7 hours, and the obtained HA dry powder is 3.6g, the glucuronic acid purity is about 44.5%, the molecular weight is 1.76MDa, protein / HA (g / g)=0.06%.
Embodiment 3
[0028] 1) Take 1000ml of fermented broth, containing 4g of HA, with a molecular weight of 2.26MDa.
[0029] 2) The temperature of the fermentation broth was raised to 110° C., maintained for 15 minutes, and filtered to obtain a filtrate.
[0030] 3) The filtrate was diluted to 3000 ml with purified water, 40 g / L of activated carbon and 2 g / L of diatomaceous earth were added, stirred and adsorbed for 5 hours, and filtered to obtain the filtrate.
[0031] 4) Add 500 g of D113 resin to the filtrate, stir and absorb for 1 hour, then filter off the resin to obtain the filtrate.
[0032] 5) Add sodium chloride to the filtrate to make the final concentration 0.4mol / L, use 2-3 times of ethanol to precipitate HA, then wash, solidify and dry to obtain powdered HA.
[0033] The whole process took 9 hours, and the obtained HA dry powder was 3.7g, the purity of glucuronic acid was about 43.8%, the molecular weight was 1.70MDa, protein / HA (g / g)=0.09%.
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