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A kind of Bacillus licheniformis and its application

A technology of Bacillus licheniformis and microbial strains, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems such as technical difficulties in biological treatment, and achieve the effect of strong temperature tolerance

Inactive Publication Date: 2016-10-05
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these reported aerobic denitrifying bacteria grow at room temperature (25-35°C), and there are few reports on aerobic denitrifying bacteria at high temperature (above 40°C).
Since the temperature of a lot of industrial wastewater reaches above 45°C, the temperature needs to be lowered before treatment, which brings great difficulties to the conventional biological treatment technology.

Method used

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  • A kind of Bacillus licheniformis and its application
  • A kind of Bacillus licheniformis and its application
  • A kind of Bacillus licheniformis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation, purification and identification of strains

[0026] In this study, a piece of filler with biofilm was taken from the reactor of the bio-trickling filtration system established by South China University of Technology in Guangzhou Ruiming Power Co., Ltd., and put into a conical flask filled with 90mL sterilized denitrification culture solution and glass beads. , shake until the biofilm falls off, and remove the filler. Then put the triangular flask into an air shaker to fully shake the biofilm to break it up. After the crushed biofilm was shaken at an initial temperature of 30°C and shaken at 160r / min for 48 hours, 10% (volume ratio) of the bacterial solution was added to 100mL of fresh sterilized denitrification culture solution, and the temperature was raised to 35°C, shaking culture on a shaking table for 48 hours, inoculate 10% (volume ratio) of the bacteria solution into 100mL of fresh sterilized denitrification culture solution, and screen the dominant b...

Embodiment 2

[0030] Detection of denitrification performance of Bacillus licheniformis JH8

[0031] Denitrification performance of Bacillus licheniformis JH8 under different carbon sources

[0032] Investigate the strain's utilization of sodium succinate, sodium citrate, glucose, potassium sodium tartrate, sodium acetate and sucrose as carbon sources. The specific operation steps are as follows:

[0033] Inoculate 5mL of bacterial suspension into 95mL of denitrification medium. The composition of denitrification medium is as follows (g / L): KNO 3 1;Na 2 HPO 4 ·7H 2 O7.9; KH 2 PO 4 1.5; MgSO 4 ·7H 2 O0.1; trace element solution 2mL / L; pH7.0~7.5. Trace element solution composition (g / L): EDTA50.0; ZnSO 4 2.2; CaCl 2 5.5; MnCl 2 4H 2 O5.06; FeSO 4 ·7H 2 O5.0; (NH 4 ) 6 Mo 7 o 2 4H 2 O1.1; CuSO 4 ·5H 2 O1.57; CoCl 2 ·6H 2 O1. 61. Five kinds of carbon sources were added to make the C / N ratio 10, and the culture was shaken at 50°C and 200r / min.

[0034] Take 0h and 24h s...

Embodiment 3

[0046] Flue gas denitrification experiment of Bacillus licheniformis JH8

[0047] flue gas biological filtration denitrification system such as Image 6 As shown, the main part is a 2.5L bio-trickling filter tower 11, which is made of plexiglass and filled with honeycomb ceramic filler 12 (porosity 0.55, diameter 4-6mm). Use the dust-removed and desulfurized flue gas to adjust the air intake, then pass through the washing tower 1, and adjust the flue gas temperature at about 50°C through the washing tower 1, and spray the cooling water in the washing tower. After that, the flue gas will flow at a certain flow and velocity It enters the biological trickling filter tower from the bottom, and then discharges out after biological filtration, and finally completes the biological filtration and denitrification process of flue gas. A microbial activity regeneration system is built outside the filter tower. The water body between the filter tower and the microbial activity regenerati...

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Abstract

The invention discloses Bacillus licheniformis and an application thereof. The Bacillus licheniformis JH8 is collected by China General Microbiological Culture Collection Center, with the collection number is CGMCC No. 8122 and the collection date is September 3, 2013. The optimal culture conditions of the strain are as follows: the temperature is 50-55 DEG C, the C / N is 12-16 and the rotational speed is 160r / min. The removal rate of the strain against nitrate radicals at the initial concentration of 140mg / L within 24h is above 95%, and the removal rate against total nitrogen is above 40%. The finding of the strain has important significance for biological denitrification and denitration under high temperature conditions.

Description

technical field [0001] The invention belongs to the field of environmental engineering and biology, and relates to the screening of a Bacillus licheniformis JH8 strain with aerobic denitrification performance and its removal of nitrate nitrogen and nitrite in waste water under high temperature environment Application of salt nitrogen and ammonia nitrogen. Background technique [0002] Due to the application of chemical fertilizers in the environment, the discharge of garbage and manure irrigation, the discharge of industrial nitrogen-containing wastewater, and the discharge of nitrogen-containing waste gas from fuel combustion, nitrate is formed after leaching and decomposition by precipitation, which flows into rivers and lakes and seeps into the ground, resulting in nitrate in surface water and groundwater. Pollution not only destroys the ecological environment of water bodies, restricts economic development, but also seriously endangers human health. The biological denit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/54C12R1/10
CPCY02A50/20
Inventor 黄少斌赵惊鸿张永清党志谭奇峰李冬平王晶惠
Owner SOUTH CHINA UNIV OF TECH
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