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An Achromobacter strain resistant to the toxicity of sulfonamide antibiotics and its application

An achromobacter, sulfonamides technology, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of reducing denitrification enzyme activity, affecting the normal operation of sewage treatment system, reducing the abundance of denitrifying microbial community, etc. Convenient operation, high-efficiency aerobic denitrification capacity, and the effect of huge economic benefits

Active Publication Date: 2020-09-25
PEKING UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that in sewage biological treatment systems, the presence of sulfonamide antibiotics has a significant negative effect on traditional anaerobic denitrification, which can not only inhibit the growth of denitrifying bacteria and their denitrification rate, but also reduce the activity of denitrifying enzymes. Moreover, it can change the structure of the denitrifying microbial community and reduce the abundance of the denitrifying microbial community, thereby affecting the normal operation of the sewage treatment system.

Method used

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  • An Achromobacter strain resistant to the toxicity of sulfonamide antibiotics and its application
  • An Achromobacter strain resistant to the toxicity of sulfonamide antibiotics and its application
  • An Achromobacter strain resistant to the toxicity of sulfonamide antibiotics and its application

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1. Utilize scanning electron microscope to observe the apparent form of achromobacter when sulfonamide antibiotic exists

[0028] The high-efficiency aerobic denitrification bacterial strain used in the method is Achromobacter sp., which is isolated from a landfill leachate treatment system.

[0029] Inoculate Achromobacter sp. into 1L of LB medium (containing 5g of NaCl, 10g of tryptone, and 5g of yeast extract per liter) to prevent the invasion of miscellaneous bacteria and maintain the growth vigor of the bacteria for enrichment to cultivate. Centrifuge the cultured bacterial liquid, wash three times with 0.5% NaCl, and make the optical density OD 600 For 1-2 bacteria suspension. Then the bacterial suspension was inoculated respectively in the LB medium containing 0 and 8 μg / L SMX by 10% inoculum size, and after 8 hours, the sample was removed by centrifugation for 5 min at 8000 rpm and 4° C., and washed with PBS (phosphate buffer) and washed twice. Fi...

Embodiment 2

[0031] Example 2. The aerobic denitrification characteristics of Achromobacter in blank control group (0 μg / L SMX)

[0032] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005g FeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0033] The strain Achromobacter sp. was inoculated in BM medium, and cultured with shaking at 30°C and 150 rpm, and 100 μL of headspace gas was extracted every 5 hours for the determination of N 2 O, 2 mL of gas was extracted with a sterile syringe and injected into a 2 L pure helium bag for the determination of NO. At the same time, 2 mL of bacterial suspension was extracted with a sterile syringe, and the bacterial liquid was centrifuged at 8000 rpm for 5 min at 4°C, and the supernatant was taken for analysis of the concentratio...

Embodiment 3

[0035] Example 3. Aerobic denitrification characteristics of Achromobacter in the presence of 2 μg / L SMX

[0036] The strain Achromobacter sp. was inoculated in BM medium containing 2 μg / L SMX to test its aerobic denitrification performance. The result is as image 3 As shown, 100 mg / L nitrate nitrogen was utilized by the strain immediately after inoculation and was completely consumed within 20 h, with an average removal rate of 5.0 mg / L / h nitrate nitrogen. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 46.7mg / L at 10h, and then was reduced at 20h. At the same time, N 2O also accumulated gradually, reaching the highest value of 10.6mg / L at 20h, and then completely reduced at 35h. It must be pointed out that the highest NO accumulation in the intermediate product of the denitrification process is 9.6 μg / L, accounting for only 0.0096% of the nitrate nitrogen removal. It can be seen that the achromobacter can s...

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Abstract

The invention relates to a strain of Achromobacter sp. resistant to the toxicity of sulfonamide antibiotics and its application in sewage treatment. The invention provides a high-efficiency aerobic denitrification bacterial strain Achromobacter sp. with tolerance to sulfonamide antibiotics, and its preservation number is CGMCC №2964. When sulfonamide antibiotics exist in the water body, the Achromobacter can still maintain a complete cell structure and efficient aerobic denitrification performance. The strain can use nitrate nitrogen as the sole nitrogen source, and under aerobic conditions, the removal rate of nitrate nitrogen can reach 100%, and the removal rate of total nitrogen can also reach 90-100%. The invention has the advantages of convenient operation and high denitrification efficiency, and has great significance for optimizing sewage biological treatment system and weakening the influence of sulfonamide antibiotics on sewage denitrification system.

Description

technical field [0001] The invention belongs to the technical field of sewage biological treatment, and in particular relates to an achromobacter which can withstand the toxicity of sulfonamide antibiotics under aerobic conditions and can simultaneously remove nitrate nitrogen in water bodies and its application in sewage treatment. Background technique [0002] With the widespread use of antibiotics in animal husbandry and aquaculture, a variety of antibiotics, such as sulfonamides, tetracyclines, macrolides, quinolones, etc., have been frequently detected in the influent water of urban sewage treatment plants in China , wherein the concentration range of sulfa antibiotics is between 20ng / L and 10μg / L. Studies have shown that most antibiotics have a broad antibacterial spectrum, which can kill certain species of microorganisms in the environment or inhibit the growth and reproduction of certain microorganisms, destroying the diversity of the ecological environment. Microor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C02F101/38C12R1/025
CPCC02F3/34C02F2101/163C02F2101/40C12N1/205C12R2001/025
Inventor 倪晋仁桂梦瑶陈倩
Owner PEKING UNIV
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