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Pseudomonas citronellolis WXP-4 and application thereof in denitrification

A WXP-4, Pseudomonas technology, applied in the direction of bacteria, microbial-based methods, water/sludge/sewage treatment, etc., can solve the problems of limited application, low nitrification rate, time-consuming and costly, and achieve a wide range of nitric acid The effect of root concentration

Active Publication Date: 2019-04-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this tends to be time-consuming and cost-intensive due to low nitrification rates and the complexity of separating aerobic and anoxic zones
In addition, autotrophic organisms grow slowly and are sensitive to environmental factors such as dissolved oxygen (DO) content, salinity, light, and substrate concentration, which limits their application in nitrogen-containing wastewater treatment.

Method used

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  • Pseudomonas citronellolis WXP-4 and application thereof in denitrification
  • Pseudomonas citronellolis WXP-4 and application thereof in denitrification
  • Pseudomonas citronellolis WXP-4 and application thereof in denitrification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation, purification and identification of Pseudomonas citronellolis WXP-4

[0028] 1. Isolation and purification of Pseudomonas citronellolis WXP-4

[0029] Pseudomonas citronellolis WXP-4 is sieved from the aerobic sludge tank of Qige sewage treatment plant. The specific steps are as follows:

[0030] The enrichment medium preparation method is as follows: Sodium succinate 2840mg L -1 , NaNO 2 345mg·L -1 , (NH 4 ) 2 SO 4 270mg·L -1 , KH 2 PO 4 1360mg·L -1 , MgSO 4 ·7H 2 O 190mg·L -1 , yeast extract 1000 mg·L -1 , trace elements 1ml·L -1 , pH=7.0, agar 20g·L -1 , the solvent is deionized water, pH=7.0.

[0031] DM Liquid Selection Medium: NaNO 3 610mg·L -1 , sodium succinate 2360mg·L -1 , KH 2 PO 4 1500 mg·L -1 , MgSO 4 ·7H 2 O 100mg·L -1 , Na 2 HPO 4 12H 2 O 7715mg·L -1 , trace elements 2ml·L -1 , agar 20g·L -1 , the solvent is deionized water, pH=7.0.

[0032] The final concentration of trace elements is composed of: ED...

Embodiment 2

[0041] Embodiment 2 Pseudomonas lanceolol WXP-4 seed culture solution

[0042] (1) Slant culture: Inoculate Pseudomonas luteolus WXP-4 into BTB medium (same composition as in Example 1), and culture at 30°C for 1 day to obtain slant cells;

[0043] (2) Seed culture: Pick colonies from the slant and inoculate them into LB medium, culture at 30°C for 12 hours, and obtain OD 600 It is 1.5 seed solution, which is the bacterial suspension; the final concentration of the LB medium is composed of: NaCl 10g / L, peptone 5g / L, beef extract 10g / L, solvent is deionized water, pH value 7.0.

Embodiment 3

[0044] Example 3: Detection of denitrification performance of Pseudomonas luteolus WXP-4

[0045] 1. Investigate the reduction of NO by Pseudomonas luteolin WXP-4 under different carbon sources 3 - -N performance

[0046] Implementation of NO reduction by Pseudomonas luteolus WXP-4 under different carbon sources 3 - -N experiment, the results show that its best carbon source is sodium succinate, and the specific implementation is as follows:

[0047] The OD prepared by embodiment 2 method 600 The bacterium suspension of 1.5 was inoculated to 5 kinds of DM liquid selective media of different carbon sources according to volume concentration 1% inoculum (all with NO 3 - -N is the only nitrogen source), 30°C, 160rpm shaking culture for 10h, take 1mL of each culture solution and centrifuge at 10000rpm for 5min to remove the microorganisms to obtain the supernatant, and then use a UV spectrophotometer to measure its concentration at 220nm and 275nm respectively Absorbance val...

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Abstract

The invention discloses a novel strain-facultative anaerobic pseudomonas citronellis WXP-4 and an application thereof in denitrification, wherein the pseudomonas citronellis WXP-4 is preserved in China Type Culture Collection Center, the address is China, Wuhan, Wuhan University, the postal code is 430072, and the preservation number is CCTCCNO: M2018526, the preservation date is August 3rd, 2018;the reduction rate of the strain to NO3-N / NO2-N with an initial concentration of 100mg L-1 can reach 100% under aerobic and anaerobic conditions, so the discovery of the degrading bacteria has important significance for further research on biological nitrogen removal.

Description

(1) Technical field [0001] The invention relates to a facultative anaerobic pseudomonas WXP-4 with denitrification performance and its application in denitrification. (2) Background technology [0002] Nitrate may be the main nitrogen pollutant in water due to its high water solubility characteristics. High concentrations of nitrate can cause environmental problems such as eutrophication of rivers and deterioration of water sources, as well as endanger human health. The World Health Organization (WHO) recommends that the concentration of nitrate in drinking water should be lower than 10mg / L, but the concentration of nitrate in groundwater in my country is generally higher than 10mg / L, and even exceeds 30mg / L. Therefore, the remediation of nitrate is a major topic that has received extensive attention in recent years. [0003] At present, nitrate removal methods in wastewater mainly include microbial denitrification, ion exchange, electrodialysis, reverse osmosis, zero-vale...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C12N1/20C12N1/205C12R2001/38
Inventor 陈浚王孝萍朵延凯何金佳张士汉陈建孟
Owner ZHEJIANG UNIV OF TECH
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