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Strain of bacillus licheniformis and applications thereof

A Bacillus licheniformis, denitrification technology, applied in the direction of bacteria, nitrous oxide capture, water/sludge/sewage treatment, etc., can solve the problems of being easily affected by temperature fluctuations, and achieve a wide growth temperature range and high efficiency The effect of oxygen denitrification capacity

Active Publication Date: 2016-03-30
SOUTH CHINA UNIV OF TECH
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, most of these reported aerobic denitrifying bacteria grow at room temperature (25-35°C), and there are few reports on aerobic denitrifying bacteria at high temperature (above 40°C), because many industrial wastewaters currently exceed 40°C , high-temperature bacteria can directly treat high-temperature wastewater, saving costs
In addition, there are few reports of strains that can maintain high denitrification performance at 30-50 °C.
One of the disadvantages of the biological method is that it is easily affected by temperature fluctuations. Therefore, it is of practical significance to screen and isolate aerobic denitrifying bacteria with high performance in a wide temperature range (including high temperature).

Method used

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  • Strain of bacillus licheniformis and applications thereof
  • Strain of bacillus licheniformis and applications thereof
  • Strain of bacillus licheniformis and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Isolation, purification and identification of strains

[0032] In this study, the denitrification medium without carbon source was added to the corn leaf hydrolyzate, and the initial temperature was 40°C, and the shaking incubator was 160rpm. After culturing for 24 hours, the inoculum with a volume ratio of 10% was inserted into 90 mL of sterile corn leaf hydrolyzate containing denitrification medium. After repeating the above steps for 3 days, it was found that the bacterial solution grew well, and the denitrification rate reached more than 85%. After the enrichment culture, the bacterial solution was spread on the plate to isolate a single colony by the method of doubling dilution. Take 1 mL of the bacterial solution into a test tube filled with 9 mL of sterile water, mix thoroughly to obtain 10 -1 Gradient bacterial suspension, prepare 10 by analogy -2 ~10 -8 Bacterial suspensions with different gradients, in order to obtain the colony morphology th...

Embodiment 2

[0035] Embodiment 2: Denitrification Performance Determination of Bacillus licheniformis (Bacillus licheniformis) B2

[0036] 1. The denitrification performance of Bacilluslicheniformis B2 under different carbon sources

[0037] The utilization of different carbon sources by strain B2 was investigated, including five carbon sources including glucose, sucrose, sodium acetate, sodium succinate and sodium citrate. The specific operation steps are as follows:

[0038] Inoculate 10mL of bacterial suspension into 90mL of denitrification medium. The composition of denitrification medium is as follows (g / L): KNO 3 1;Na 2 HPO 4 ·7H 2 O0.8; KH 2 PO 4 1.5; MgSO 4 ·7H 2 O0.2; trace element solution 2mL / L; pH7.0. Trace element solution composition (g / L): EDTA50.0; ZnSO 4 2.2; CaCl 2 5.5; MnCl 2 4H 2 O5.06; FeSO 4 ·7H 2 O5.0; (NH 4 ) 6 Mo 7 o 2 4H 2 O1.1; CuSO 4 ·5H 2 O1.57; CoCl 2 ·6H 2 O1. 61. The above five carbon sources were added respectively to make the C / N r...

Embodiment 3

[0052] Embodiment 3: Actual flue gas denitrification experiment of Bacillus licheniformis (Bacillus licheniformis) B2

[0053] Removal of NO in High Temperature Environment Using Biological Trickling Filter x experiment of. biofiltration systems such as Figure 7 As shown, air inlet 1, atomizer humidifier 2, temperature controller 3, biological trickling filter tower 4, constant temperature water bath 5, mass flow meter 6, and gas outlet 7. The nutrient composition in the liquid storage tank during the film-forming period of the biofilter is the same as in Example 2, and the stability of the system under the condition of actual flue gas fluctuations is investigated.

[0054] The flue gas flow rate is 1L min -1 , the residence time is 1min. To keep B2 dominant at all times, replace two-thirds of the nutrient solution containing 10% fresh B2 every three days. The removal efficiency of nitrate increased gradually, and after reaching 80%, the concentration of nitrate in the n...

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Abstract

The invention discloses a strain of bacillus licheniformis and applications thereof. The bacillus licheniformis (B2) is preserved in China Center for Type Culture Collection, the preservation number of the strain is CCTCC No. M20155590, and the preservation time is October 8th, 2015. The provided strain of bacillus licheniformis has the advantages of wide growth temperature range and high aerobic denitrification performance, and can be used to process nitrate wastewater or flue gas containing NOx. The provided strain can be used to process nitrate with a primary concentration of 140 mg / L at a temperature of 30 to 50 DEG C, within 24 hours, the nitrogen elimination rate can reach 99% or more, and at the same time no nitrite is accumulated. The strain can solve the problem that the biological denitration and denitrogenation are severely influenced by the temperature fluctuation.

Description

technical field [0001] The invention belongs to the fields of environmental engineering and biology, and relates to a Bacillus licheniformis B2 strain with aerobic denitrification performance and its application in removing nitrate and nitrite in waste water under high temperature environment. Background technique [0002] Major changes have taken place in agricultural production methods, and the use of pesticides and chemical fertilizers is increasing day by day. Pesticides and fertilizers are applied to crops, 10% to 20% of which are attached to the plants, and the remaining 80% to 90% are scattered in the soil, water, and atmosphere. Pesticides and fertilizers enter surface water bodies through surface runoff or precipitation, causing eutrophication and even polluting groundwater. Dyeing and textile wastewater, oil refining wastewater, meat processing and feed production wastewater all contain high concentrations of ammonia nitrogen and nitrate. In addition, due to the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34B01D53/84B01D53/56C12R1/10C02F101/16
CPCB01D53/56B01D53/84B01D2251/95B01D2257/402B01D2257/404C02F3/02C02F3/34C02F2101/163C02F2101/166C12N1/205C12R2001/10Y02C20/10Y02W10/10Y02A50/20
Inventor 黄少斌何嘉欣周少锋
Owner SOUTH CHINA UNIV OF TECH
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