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Preparation and application of complex microbial inoculant for environment-friendly ecological toilet

A compound bacterial agent and ecological technology, applied in the field of microorganisms, can solve the problems of potential risks to the human body and the environment, low biodiversity index, low deodorization efficiency, etc. The effect of germ growth

Active Publication Date: 2022-05-27
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the microbial agents that have been applied, such as the invention patent of Chinese Patent Application Publication No. CN110157644A, disclose that through the combination of Bacillus flexus and Bacillus megaterium, it can fix sulfur and nitrogen and effectively remove odorous and toxic hydrogen sulfide. , ammonia; but its biodiversity index is low, the composition is single, it is difficult to play a role in a short time, and the deodorization efficiency is not high
For example, the Chinese patent application publication number is CN106967640A, which discloses that the mutual cooperation of Pseudomonas and Bacillus and comprehensive treatment of stench can achieve a better deodorizing effect than that of a single bacterial species; Opportunistic pathogenic bacteria with potential risks to humans and the environment

Method used

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  • Preparation and application of complex microbial inoculant for environment-friendly ecological toilet
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Experimental program
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preparation example Construction

[0038] The preparation process of the composite bacterial agent of the present invention is as follows:

[0039] (1) Pediococcus lactis WP is inserted into the MRS solid slant culture for activation for subsequent use, and Bacillus velesi WB is inserted into the beef extract peptone solid slant medium for activation for subsequent use;

[0040] (2) The activated Pediococcus lactis WP was inserted into the MRS liquid medium, and the Bacillus velesi WB was inserted into the beef extract peptone liquid medium, and the culture was shaken for 24 hours at 30°C and 160 r / min;

[0041] (3) The cultured Pediococcus lactis WP bacterial liquid and Bacillus velesi WB bacterial liquid are counted and adjusted, and the bacterial liquid concentration of each bacterial strain is adjusted to be 10 9 CFU / mL, then mix the two bacterial solutions according to the volume ratio of 1:1 to form a mixed bacterial solution, and then add 10-15% (v / v) of the prepared enzyme filtrate to finally obtain a liq...

Embodiment 1

[0047] 1. Add 10mL of liquid compound bacterial agent to the beaker (1L) containing 400g of fresh pig manure, seal the beaker with plastic wrap, and add Pediococcus lactis WP to the beaker (1L) containing 400g of fresh pig manure according to the above method. Bacterial liquid and Bacillus velesi WB bacterial liquid, only one bacterial liquid is added to each beaker. At the same time, no bacterial agent was used as the control group, and three replicates were set for each group of experiments, which were cultured in a 35°C incubator with regular stirring every day; at the same time, multiple comparison groups were set up with different bacterial species. . After 7 days, take samples to measure volatile solids VS, odor intensity value, NH 3 Concentration and H 2 S concentration. The degradation performance of the bacterial agent is measured according to the change of VS, according to the odor, NH 3 Concentration and H 2 The change of S concentration is used to measure the ...

Embodiment 2

[0071] In Chengdu Qinglong Lake Wetland Park, four ecological toilets were selected, and 5kg of filler and 500g of solid compound microbial inoculants (after activation) were added to them, and the devices were started and put into use; compared with other ecological toilets without inoculants. Samples were taken after 7d, 14d, 21d and 30d to measure volatile solid VS, odor intensity value, NH 3 Concentration and H 2 S concentration, take the average value. The degradation performance of the bacterial agent was evaluated according to the change of VS, according to the odor, NH 3 Concentration and H 2 The change of S concentration was used to evaluate the deodorizing effect of the bacterial agent. The measurement results are shown in Table 6 below.

[0072] Table 6 Degradation and deodorization effect of bacterial agent

[0073]

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Abstract

The invention relates to the technical field of microorganisms, in particular to preparation and application of a complex microbial inoculant for an environment-friendly ecological toilet. According to the specific technical scheme, the complex microbial inoculant for the environment-friendly ecological toilet comprises pediococcus acidilactici and bacillus velezensis, and the complex microbial inoculant can well grow in ecological side seat excrement, occupies a favorable ecological niche, inhibits growth and reproduction of microorganisms producing stink, reduces the stink release intensity and realizes stink in-situ source control. Meanwhile, manure decomposition and degradation are promoted.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the preparation and application of a composite bacterial agent for an environment-friendly ecological toilet. Background technique [0002] Due to the large population in my country and the huge volume of human excrement and urine produced every day, how to solve the problem of excrement and its odor is a common problem faced by everyone. However, most urban residents in my country use water to flush toilets in their homes, and human waste and urine enter the sewage treatment plant through the urban domestic sewage discharge pipe network for treatment. However, the flush toilet not only increases the amount of manure, but also increases the difficulty of harmless treatment after the manure is diluted, which is not conducive to the reuse of manure resources. Some old urban areas and rural areas, especially in the alpine and desert areas of our country, are extremely scarce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F11/02C12R1/07C12R1/225C12R1/01
CPCC12N1/20C12N1/16C02F11/02C02F2303/02Y02A50/30
Inventor 闫志英王茄灵许力山刘杨姬高升
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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