Vaccine for prevention of toxoplasma infection as well as preparation method and application of vaccine

A technology for infection prevention and Toxoplasma gondii, which is applied in the fields of immunology and molecular biology, can solve the problems of high technical difficulty, weak anti-infection ability, formation of tissue cysts, etc., and achieve the effect of prolonging survival time and reducing the amount of worms

Inactive Publication Date: 2014-07-23
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The safety of inactivated vaccine is better, but its anti-infection ability is weak; the attenuated vaccine has better protection, but in terms of safety, there has always been a strong virulence and the formation of tissue packets in the body after Toxoplasma infection of the intermediate host. The production process of genetically engineered vaccines is com...

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  • Vaccine for prevention of toxoplasma infection as well as preparation method and application of vaccine
  • Vaccine for prevention of toxoplasma infection as well as preparation method and application of vaccine
  • Vaccine for prevention of toxoplasma infection as well as preparation method and application of vaccine

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Embodiment Construction

[0034] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0035] In the present invention, experimental materials are purchased from:

[0036] Female KM mice, about 6 weeks old, weighing 20±2g, were purchased from the Medical Experimental Center of Lanzhou University.

[0037] Host bacteria, Toxoplasma gondii RH, PRU strains, pVAX I eukaryotic expression vector and other materials were all preserved and provided by the Laboratory of Parasite Functional Genomics, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultu...

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Abstract

The invention provides a nucleotide sequence for prevention of toxoplasma infection. The nucleotide sequence for prevention of toxoplasma infection contains nucleotide sequences selected from (1) a nucleotide sequence as shown in SEQ ID No.1 of a sequence table, (2) a nucleotide sequence complementary with the nucleotide sequence as shown in the (1), and (3) a nucleotide sequence obtained by replacing, deleting, adding and modifying one or multiple of basic groups of the nucleotide sequence as shown in the (1) or (2) and having a function of preventing toxoplasma infection. The invention also provides a vaccine which comprises the nucleotide sequence and a medically acceptable carrier. The plasmid DNA vaccine pVAX-ROP38 can be used for effectively preventing and controlling infection of toxoplasma animal models (Kunming strain mouse), namely can be used for effectively prolonging the survival time of the mouse and reducing the worm burden of brain capsules. Therefore, the plasmid pVAX-ROP38 can serve as a DNA vaccine candidate antigen for resisting toxoplasma infection.

Description

technical field [0001] The invention belongs to the fields of immunology and molecular biology, and in particular relates to a vaccine for preventing toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii (T.gondii) is an obligate intracellular parasitic protozoan that can cause zoonotic toxoplasmosis. Toxoplasma gondii can infect almost all mammals including humans, and cats are the main source of infection. About 1 / 3 of the adults in the world are infected, the infection rate of the Chinese population is 5% to 20%, and the infection rate of livestock animals is 10% to 50%. As an opportunistic pathogenic factor, Toxoplasma gondii is usually recessively infected in normal hosts without obvious clinical symptoms; but for organ transplantation, AIDS, malignant tumor patients and other immunodeficiency or immunosuppression patients, Toxoplasma gondii may be fatal. One of the factors; Toxoplasma gondii infection in pregnant women can lead to miscarriage, ...

Claims

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Application Information

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IPC IPC(8): C12N15/52C12N9/00C12N15/85A61K48/00A61K39/002A61P33/02
Inventor 黄思扬徐颖张念章徐前明徐民俊宋慧群朱兴全
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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