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Method for detecting resistance marker in growing period of atractylodes macrocephala

A marker, Atractylodes macrocephala technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as hindering the discovery of Atractylodes macrocephala

Inactive Publication Date: 2014-07-23
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

However, Atractylodes macrocephala, like other medicinal plants, lacks a large amount of sequence data information, making it difficult to apply systematic analysis of gene expression, transcriptional profiling analysis methods such as microarrays, and cDNA-ALFP technology to the expression analysis of genes related to various biological functions. Biological functions include heredity, fertility, and disease resistance of Atractylodes macrocephala, which seriously hinders the large-scale gene discovery of Atractylodes rhizome

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  • Method for detecting resistance marker in growing period of atractylodes macrocephala
  • Method for detecting resistance marker in growing period of atractylodes macrocephala
  • Method for detecting resistance marker in growing period of atractylodes macrocephala

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Embodiment

[0018] Concrete steps in this embodiment are:

[0019] A field natural sample collection experiments were carried out in Changhua, Zhejiang and Tianmushan medicinal planting bases in Zhejiang. Atractylodes macrocephala was planted in mid-March, 2010, and during the peak period of the incidence of white silkworm in mid-June, 2011, the susceptible disease (A 1 ) and resistant plants (A 2 ) each 30 strains were removed from soil, dried at low temperature, and used as test samples for subsequent use.

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Abstract

The invention discloses a method for detecting a resistance marker in the growing period of atractylodes macrocephala. The method comprises: in the soil borne disease high-incidence season of atractylodes macrocephala, collecting infected plants and disease-resistant plants in natural state; also, employing a root cutting method to inoculate with sclerotium rolfsii mycelium; after the plants are infected and attacked, respectively collecting healthy plants and disease plants, and employing HPLC method to determine the level of main metabolites atractylenolides in the sample plants; comparing differences in groups, determining a significant-difference composition, and screening a metabolite marker correlated to atractylodes macrocephala resistance; and taking sclerotium rolfsii Sacc. as a test-receiving bacteria, and performing virulence tests on the composition, so as to prove the bacteriostatic efficacy of atractylenolide II and further to obtain the reisistance marker which is used for atractylodes macrocephala resistance evaluation, resistance variety breeding and other aspects.

Description

technical field [0001] The invention relates to a method for detecting resistance markers in the growth stage of Atractylodes macrocephala, in particular to a secondary metabolite atractylodes lactone II used as a marker detection method related to the resistance of Atractylodes rhizome soil-borne diseases. Background technique [0002] Atractylodes macrocephala (Atractylodis macrocephala Koidz.) is one of the bulk Chinese herbal medicines in my country. It has been introduced and cultivated in about 20 provinces (municipalities) across the country, and the current planting area is about 13,000 hectares. However, under the conditions of suitable soil conditions, cultivation conditions and climate conditions, the loss rate of Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma is as high as 50% when serious, and even cause crop failure. Soil-borne diseases of Atractylodes macrocephala are the limiting f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 田薇王春荣李倩蒋鹏
Owner ZHEJIANG FORESTRY UNIVERSITY
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