Formula and preparation method of large yellow croaker lactobacillus plantarum feed

A technology of Lactobacillus plantarum and feed formula, which is applied in the field of Lactobacillus plantarum feed formula and its preparation in large yellow croaker, can solve the problems of insecure food safety, poor immunity and quality, slow growth rate, etc., and achieve the promotion of weight growth rate and Effects of food intake rate, strong immunity, and fast growth

Inactive Publication Date: 2014-07-30
WENZHOU UNIVERSITY
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Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a large yellow croaker Lactobacillus plantarum feed formula and its preparation method, aiming to solve the problems of slow growth, high disease rate, poor immunity and quality, and low survival rate in traditional large yellow croaker feed. , food safety is not guaranteed

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  • Formula and preparation method of large yellow croaker lactobacillus plantarum feed
  • Formula and preparation method of large yellow croaker lactobacillus plantarum feed
  • Formula and preparation method of large yellow croaker lactobacillus plantarum feed

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preparation example Construction

[0030] Such as figure 1 Shown, the preparation method of the large yellow croaker plantarum lactobacillus feed of the embodiment of the present invention comprises the following steps:

[0031] S101: Lactobacillus plantarum was isolated from Korean pickled cabbage, cultivated in a sterile flask containing MRS broth at 37°C, and centrifuged at 15400×g for 5 minutes after 24 hours to collect the Lactobacillus plantarum mixture;

[0032] S102: Mix it with skim milk at a ratio of 1:4, freeze-dry, and measure the viability of the Lactobacillus plantarum mixture by counting on the MRS agar plate;

[0033] S103: According to Lactobacillus plantarum 1×10 6cfu / kg, bacteria 0.001g / kg, milk 9.999g / kg, fish meal 540g / kg, starch 183g / kg, fish paste 37g / kg, gluten 20g / kg, fish oil 20g / kg, shrimp shell powder 64g / kg, Defatted soybean meal 94g / kg, mineral mixture 35g / kg, vitamin mixture 7g / kg configure large yellow croaker feed;

[0034] S104: Add a basic feed with ingredients of 47.3% cru...

Embodiment 1

[0037] 1 Materials and methods:

[0038] 1.1 Preparation of Lactobacillus plantarum mixture feed:

[0039] Lactobacillus plantarum Saccharomycescerevisiae P13 isolated from Korean pickled cabbage [2] , cultured in a sterile flask containing MRS broth at 37°C, centrifuged at 15,400×g for 5 minutes after 24 hours, collected the Lactobacillus plantarum mixture, mixed it with skim milk at a ratio of 1:4, freeze-dried, and recorded on the MRS agar plate As shown in Table 1, five kinds of large yellow croaker feeds were configured according to the content of Lactobacillus plantarum, wherein the basic feed ingredients were 47.3% crude protein, 8.9% crude fat, 10.2% ash and 8.4% Moisture, 1.0×10 9 The cfu / g Lactobacillus plantarum mixture was added to the basal feed in the ratio shown in Table 1, so that the content of Saccharomyces cerevisiaeP13 Lactobacillus plantarum in the feed for large yellow croaker reached 0 and 10 respectively. 4 、10 6 、10 8 、10 10 cfu / kg;

[0040] 1.2...

Embodiment 2

[0063] 1 Materials and methods:

[0064] 1.1 Large yellow croaker breeding:

[0065] Large yellow croaker juveniles were purchased from a local artificial farm, and the feeding experiment was carried out in a tank with running water (sea water). During the experiment, 30% of the sea water was replaced every day to ensure the water quality, and the water temperature was kept at 28±0.2°C , the pH value of the water is 7.5-8.4, and the salinity of the water is maintained at 2.0 ± 0.1%. The plantarum Lactobacillus SaccharomycescerevisiaeP13 is isolated from Korean pickled cabbage and cultivated in a sterile flask containing MRS broth at 37°C. After 24 hours, Centrifuge at 15,400×g for 5 minutes to collect the Lactobacillus plantarum mixture, mix it with skim milk at a ratio of 1:4, freeze-dry, and measure the viability of the Lactobacillus plantarum mixture by counting on the MRS agar plate. All experimental fish are prepared according to Table 1 every day. The formula feed or th...

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Abstract

The invention discloses a formula and a preparation method of large yellow croaker lactobacillus plantarum feed. The formula of the large yellow croaker lactobacillus plantarum feed comprises feeding feed and basal feed, wherein the feeding feed comprises 1*10<6> cfu/kg of lactobacillus plantarum, 0.1g/kg of thallus, 9.999g/kg of milk, 540g/kg fish meal, 183g/kg of starch, 37g/kg of fish paste, 20g/kg of gluten, 20g/kg of fish oil, 64g/kg of shrimp shell powder, 94g/kg of defatted soybean meal, 35g/kg of mineral mixture and 7g/kg of vitamin mixture, the percentage by mass of the feeding feed is 25.2%, and the basal feed comprises 47.3% of crude protein, 8.9% of crude fat, 10.2% of ash content and 8.4% of moisture content in percentage by mass. The preparation method comprises the following steps: carrying out separation culture, collecting the lactobacillus plantarum, mixing the defatted milk, adding the basal feed, mixing and preparing. The large yellow croaker lactobacillus plantarum feed can be used for increasing growth rate of body mass of large yellow croakers and feeding rate of the large yellow croakers, improving non-specific immunity performances such as complement activity, lysozyme activity, phagocytic activity and respiration of leukocytes in head and kidney of the large yellow croakers and enhancing pathogenic bacteria resistance and facilitates development of large yellow croaker breeding industries.

Description

technical field [0001] The invention belongs to the technical field of large yellow croaker breeding, in particular to a large yellow croaker Lactobacillus plantarum feed formula and a preparation method thereof. Background technique [0002] Large yellow croaker is a unique fish with high economic value in my country, and it is one of the most valuable species in marine fish farming in China. In recent years, the breeding scale of large yellow croaker has rapidly expanded due to the success of artificial breeding. Due to overfishing, natural resources are increasingly depleted. After the success of artificial breeding, the scale of large yellow croaker cages expanded rapidly. The unreasonable planning of cage culture has led to a sharp increase in the breeding area and stocking density, which has seriously endangered the carrying capacity of the breeding waters and the self-purification capacity of water exchange, and the types of fish diseases have also increased, which g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/10A23K1/08A23K1/14A23K1/175A23K10/18A23K10/20A23K10/22A23K10/26A23K10/30A23K10/37A23K20/158A23K20/174A23K20/20A23K50/80
CPCY02A40/818Y02P60/87
Inventor 廖志勇缪雄伟林利李小花
Owner WENZHOU UNIVERSITY
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