A sandwich-type multi-layer immobilized bioactive filler of sulfur-oxidizing bacteria, its preparation and application
A sulfur-oxidizing bacteria and biologically active technology, applied in the field of water treatment, can solve the problems of poor overall stability of biologically active fillers, unsatisfactory effects of biologically active fillers, and easy detachment of embedded bodies, so as to achieve full contact and reduce dense film The formation and the effect of good fluidization state
Active Publication Date: 2016-10-19
湖南汨罗循环经济产业园区科技创新服务中心
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Problems solved by technology
After experimental research and comparison, we found that the bioactive filler prepared based on the traditional filler structure and embedding method did not perform well in the experimental application, and there were many problems such as poor overall stability of the bioactive filler, easy detachment of the embedded body, and water solubility.
Method used
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Embodiment 1
[0024] (1) Centrifuge and concentrate the sulfur-oxidizing bacteria after amplification and culture, take 45L of centrifuged, and the bacteria content after centrifugation is 3.5×10 8 The concentrated solution of sulfur-oxidizing bacteria per mL is for use;
[0025] (2) Take 30.0kg of polyvinyl alcohol in a water bath and heat it to 90°C and dissolve it in 200L of water to obtain a polyvinyl alcohol solution;
[0026] (3) Cool the polyvinyl alcohol solution in step (2) to 30°C and fully mix it with the bacterial concentrate obtained in step (1), add water to make up to 300L to obtain an embedding solution, and the polyvinyl alcohol in the embedding solution The mass concentration is 100g / L;
[0027] (4) Spread the embedding solution obtained in step (3) evenly on the polyvinyl alcohol non-woven fabric carrier, superimpose four layers of carriers together, take it out after 1.5 hours of crosslinking with saturated boric acid, and adjust the pH value of the boric acid solution ...
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Abstract
A sulfur-oxidizing bacterium sandwich-type multilayer immobilized bioactive filler, preparation and application belong to the field of water treatment. The bioactive filler comprises two parts, embedding bodies and non-woven fabric carriers. The embedding liquid comprises a mixture of a sulfur-oxidizing bacterium concentrate, and a polyvinyl alcohol solution; the non-woven fabric carriers are coated uniformly with the embedding liquid; several layers of the carriers are overlapped together; after secondary crosslinking fixing by boric acid, a layered filler with a sandwich-like structure is formed; the layered filler is cut to obtain a granular bioactive filler. Fibers of the non-woven fabric carriers can bond with the embedding bodies stably; the alternate arrangement of the embedding bodies and the non-woven fabric carriers improves the stability of the bioactive filler. The sulfur-oxidizing bacterium sandwich-type multilayer immobilized bioactive filler prepared in the invention can not only effectively solve the problems of poor adhesion and easy loss of sulfur-oxidizing bacteria, but also improve the processing capacity of reactors, shorten the starting time, and thus has good application prospects.
Description
technical field [0001] The invention belongs to the field of water treatment, and in particular relates to the preparation and application of a sandwich type multi-layer immobilized bioactive filler of sulfur oxidizing bacteria. Background technique [0002] With the rapid development of my country's industry, a large amount of sulfide-containing wastewater is often discharged in oil refining, coking, pharmaceutical, and petrochemical industries. At present, the treatment of sulfide in water generally includes physical and chemical methods and biological oxidation methods, and the biological oxidation method has gradually become a sulfur-containing method due to its advantages such as no need to add chemicals, high removal efficiency, less biological sludge generation, and no secondary pollution. The main methods and research focus of wastewater treatment. When using the biological metabolic function of sulfur-oxidizing bacteria to treat sulfur-containing wastewater, the me...
Claims
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IPC IPC(8): C12N11/08C02F3/10C02F3/34C02F3/00
CPCY02W10/10
Inventor 杨宏赵月兰陈伟管清坤鄢琳王猛王小乐吴城锋孟婷陶慕翔尚海源胡希佳王玉洁姚仁达
Owner 湖南汨罗循环经济产业园区科技创新服务中心