Culture solution for cell source for bioartificial liver

Abstract

The invention discloses a culture solution for a cell source for a bioartificial liver. The culture solution comprises a mixed solution comprising a low-sugar type DMEM culture solution and fetal bovine serum according to the volume ratio of (8-12): 1, wherein the mixed solution further contains 60-140U / L of insulin, 3-9mu g / L of hepatocyte growth factor, 0.5-2g / L of magnesium isoglycyrrhizinate, 3-10mg / L of tauroursodeoxycholic acid and 50-100U / ml of green chain double-antibody. The culture solution for the cell source for the bioartificial liver disclosed by the invention can promote the growth of hepatocytes or hepatocyte-like cells, is conductive to developing the cells to the direction of better realizing liver functions, particularly can effectively improve the survival rate and the survival time of the cells in a hyperbilirubinemia environment and improve the curative effect by improving an existing DMEM culture medium and adding insulin for protecting the hepatocytes, hepatocyte growth factor, magnesium isoglycyrrhizinate and tauroursodeoxycholic acid. The culture solution is suitable for culturing various hepatocytes and hepatocyte-like cells, in particular to L-02 cells.

Description

technical field [0001] The invention relates to the technical field of hepatocyte and hepatocyte-like culture, in particular to a cell-source culture solution for a bioartificial liver. Background technique [0002] my country is the country with the most serious liver disease in the world, with more than 1 million new cases of liver failure each year, and about 500,000 liver failure patients die each year due to lack of effective treatment. There is only one chance of cure, that is, liver transplantation, but due to the difficulty in obtaining livers, the long waiting time, or no chance to get a donor liver at all, most patients have no chance of survival at all. Therefore, an artificial liver system is urgently needed to maintain the patient's life, waiting for the opportunity of liver transplantation, or the opportunity of the patient's own liver recovery. [0003] However, due to the high cost of the traditional artificial liver (that is, the physical artificial liver),...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defect that the existing hepatocyte culture medium cannot resist high bilirubin toxicity and easily cause apoptosis of hepatocytes or hepatocyte-like cells in the artificial liver system, and provide a bioartificial bioartificial agent capable of resisting bilirubin toxicity. Cell-derived culture medium for liver