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Biological enzyme modified natural lacquer composition and preparation method thereof

A biological enzyme and composition technology, applied in the field of chemically modified biological enzyme modified natural lacquer composition and its preparation, can solve problems such as environmental pollution

Active Publication Date: 2016-01-20
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the performance of natural raw lacquer can be improved to a certain extent through chemical modification, the defects of raw lacquer itself have not been fundamentally resolved. At the same time, the use of organic solvents in the chemical modification process is likely to cause environmental pollution. Therefore, the use of environmentally friendly biological Technical modification of natural lacquer and fundamentally improving its performance is an inevitable choice to increase the added value of natural lacquer and expand its application range

Method used

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  • Biological enzyme modified natural lacquer composition and preparation method thereof
  • Biological enzyme modified natural lacquer composition and preparation method thereof
  • Biological enzyme modified natural lacquer composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: polyethylene glycol modification white rot fungus C. cinerea laccase

[0041] 40g of PEG2000 was dissolved in 100mL of dry tetrahydrofuran, and 2.0g of succinic anhydride (SA) was added and then kept at 60°C for 2h. Then lyophilized to get PEG2000-OCOCH 2 CH 2 COOH (PEG2000-SA). PEG2000-SA, N-hydroxysuccinimide (NHS) and dicyclohexylcarbodiimide (DCC) were added into a three-necked flask in an equimolar ratio, and stirred overnight at room temperature. The precipitate was filtered off, and the filtrate was distilled. Ether washing, vacuum drying to obtain active ester PEG2000-SA-NHS (PEG2000-OCOCH 2 CH 2 COO-C 4 h 4 NO 3 ).

[0042] Active ester PEG2000-SA-NHS and white rot fungus C. cinerea laccase (CC-laccase) were fed at a mass ratio of 20:1, added to pH 3.0 citrate buffer, and reacted at 4°C for 4 hours. After lyophilization, PEG2000-modified white-rot fungus C. cinerea laccase (PEG2000-SA-NHS-CC-laccase) (number 1) was obtained.

[0043] Th...

Embodiment 2

[0045] Embodiment 2: Polypropylene glycol modification white rot fungus C.cinerea laccase

[0046] 60g of PPG2000 was dissolved in 200mL of dry tetrahydrofuran, 3.2g of succinic anhydride (SA) was added, and then kept at 65°C for 3h. Then lyophilized to get PPG2000-OCOCH 2 CH 2 COOH (PPG2000-SA), PPG2000-SA, N-hydroxysuccinimide (NHS) and dicyclohexylcarbodiimide (DCC) were added into a three-necked flask in an equimolar ratio, and stirred overnight at room temperature. The precipitate was filtered off, and the filtrate was distilled. Washing with ether, drying in vacuo to obtain active ester PPG2000-SA-NHS (PPG2000-OCOCH 2 CH 2 COO-C 4 h 4 NO 3 ).

[0047] Active ester PPG2000-SA-NHS and white rot fungus C. cinerea laccase (CC-laccase) were fed at a mass ratio of 15:1, added to citrate buffer with pH 3.0, and reacted at 10°C for 4 hours. After freeze-drying, PPG2000 modified white-rot fungus C. cinerea laccase (PPG2000-SA-NHS-CC-laccase) (number 2) was obtained.

Embodiment 3

[0048] Embodiment 3: polyethylene glycol modified ascorbate oxidase

[0049] 80g of PEG2000 was dissolved in 200mL of dry tetrahydrofuran, and 4.0g of succinic anhydride (SA) was added and kept at 55°C for 2h. Then lyophilized to get PEG2000-OCOCH 2 CH 2 COOH (PEG2000-SA). PEG2000-SA, N-hydroxysuccinimide (NHS) and dicyclohexylcarbodiimide were added into a three-necked flask in an equimolar ratio (DCC), and stirred overnight at room temperature. The precipitate was filtered off, and the filtrate was distilled. Wash with ether and dry in vacuo to obtain the active ester PEG2000-SA-NHS.

[0050] Active ester PEG2000-SA-NHS and ascorbate oxidase (Ascorbateoxidase) were fed at a mass ratio of 15:1, added to citrate buffer with pH 3.0, and reacted at 4°C for 4 hours. After lyophilization, PEG2000-modified ascorbate oxidase (PEG2000-SA-NHS-Ascorbateoxidase) (number 3) was obtained.

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Abstract

The invention relates to an NIS (nonionic surfactant) modified bio-enzyme modified natural raw lacquer composition which is characterized by comprising components A and B, wherein the component A is natural raw lacquer or a prepolymer of the natural raw lacquer and tung oil; the component B is chemical modification bio-enzyme; the additive amount of the chemical modification bio-enzyme in the component B is 1.0-10.0% and preferably 2.0-8.0% of the mass fraction of the component A; the chemical modification bio-enzyme is natural bio-enzyme modified by the NIS; the components A and B are stored in a separated manner, mixed in proportion and uniformly stirred before being painted, and painted on the surface of an object to produce raw lacquer.

Description

technical field [0001] The invention relates to a chemically modified biological enzyme modified natural lacquer composition and a preparation method thereof, in particular to a nonionic surfactant modified biological enzyme modified natural lacquer composition and a preparation method thereof. The composition is obtained by chemically modifying biological enzymes with nonionic surfactants, and then modifying natural lacquer with modified biological enzymes. Background technique [0002] Sumac is an important rare plant resource in China. my country's sumac resources are very rich and widely distributed in the central and western regions. Raw lacquer, a natural polymer material collected from the bark of the lacquer tree, is an important ecological material, whose output accounts for 85% of the world's total output, and is known as the "king of paint". Raw lacquer is mainly composed of urushiol, laccase, gum, glycoprotein, water, metal ions and other biochemical substances....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D193/00C09D7/12C08G65/48
Inventor 廖有为杨焰车轶材
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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