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A kind of preparation method of magnetic agarose microsphere

A technology of agarose microspheres and agarose, which is applied in the field of preparation of magnetic agarose microspheres, can solve the problems of uneven coating of magnetic agarose microspheres, low separation and purification, and poor magnetic response, and achieve smooth ball formation , Good dispersibility, and the effect of improving the coating rate

Active Publication Date: 2016-08-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the magnetic agarose microspheres prepared by this method have shortcomings such as uneven coating, low coating efficiency, poor spherical shape, and small specific surface area. Although the mechanical properties of the agarose have been improved, new defects have also been brought. Such as low separation and purification filter, poor magnetic response, etc.

Method used

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  • A kind of preparation method of magnetic agarose microsphere
  • A kind of preparation method of magnetic agarose microsphere
  • A kind of preparation method of magnetic agarose microsphere

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preparation example Construction

[0034] 1. the preparation method of a kind of agarose nonmagnetic microsphere of the present invention, comprises the following steps:

[0035] (1) Provide agarose aqueous solution containing one or several additives as the water phase W, and heat at a certain temperature to dissolve the agarose.

[0036] (2) Provide one or several organic solvents that are immiscible with the water phase as the oil phase O, and dissolve one or several emulsifiers in it. Heat and stir the oil phase O at a constant temperature and constant speed, add the water phase W to the oil phase O in a certain proportion, and mechanically stir the reaction at a certain speed for a certain period of time to obtain a W / O emulsion.

[0037] (3) Rapidly lower the W / O emulsion to a certain temperature to form agarose microspheres.

[0038] More specifically, in step (1), the agarose aqueous solution provided has a preset concentration, and the agarose-containing aqueous solution will be used as the aqueous ph...

Embodiment 1

[0059] 1. Preparation of non-magnetic agarose microspheres:

[0060] (1) Add 2.0 g of agarose microspheres to 100 ml of ultrapure water and heat at 100°C to prepare the agarose aqueous phase W.

[0061] (2) Add 180ml of liquid paraffin, 40ml of petroleum ether, add 8.0g of emulsifier Span 80, and fully disperse to prepare oil phase O.

[0062] (3) The oil phase O was heated at a constant temperature of 80°C, mechanically stirred at 400 rpm, and the water phase was quickly transferred in, and reacted for 3 hours.

[0063] (4) Cool the ice water bath to 15°C rapidly.

[0064] 2. Activation and cross-linking of non-magnetic agarose microspheres:

[0065] (1) Take 5g of microspheres, add 7.5ml of 0.8mol / L NaOH, 0.01g of sodium borohydride, shake at 25°C and 400rpm for 30min.

[0066] (2) Add 7.5ml of dimethyl sulfoxide and 3.75ml of epichlorohydrin to the above solution. React at a constant temperature of 30°C and 400rpm for 20h.

[0067] (3) Wash to remove unreacted reagents...

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Abstract

The invention discloses a preparation method of magnetic agarose microspheres, which comprises the preparation of agarose nonmagnetic microspheres, activation and crosslinking of agarose nonmagnetic microspheres, preparation of agarose nonmagnetic microsphere iron ion solution and chemical co-transformation Preparation of magnetic agarose microspheres. The invention improves the traditional method of coating magnetic particles with agarose, and changes the traditional direct coating method into an indirect coating method. The advantage of the present invention is that the prepared magnetic agarose microspheres are interspersed with magnetic ferric iron tetroxide core particles, which greatly increases the mechanical strength of the agarose and overcomes the soft texture and easy structure damage. , the defect of short service life, at the same time, the magnetic particles have superparamagnetism, strong magnetic response, and can achieve rapid separation under an external magnetic field; compared with the traditional method, the magnetic balls have improved spheroidization, round spheres, and are not easy to agglomerate and agglomerate. Good dispersion, uniform particle size distribution, and increased specific surface area increase the separation and purification rate.

Description

technical field [0001] The invention belongs to the technical field of magnetic organic-inorganic composite materials, and in particular relates to a preparation method of magnetic agarose microspheres. Background technique [0002] At present, the material matrices commonly used for separation and purification in the field of biomedicine can be divided into two categories: inorganic and organic. Inorganic matrix media mainly include silica gel, porous glass, hydroxyapatite, etc., which are mostly used as filling materials for high performance liquid chromatography. The medium of the organic matrix is ​​usually natural polysaccharides, synthetic polymers, etc., and the matrix prepared from these materials is usually in the shape of beads. Generally, a base sphere of a parent structure can be chemically modified to derive separation media with different functional groups, different structures, different properties, and different types. It can be seen that the base ball is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12C08J3/24C08L5/12C08K3/22B01J13/02
Inventor 赵九蓬马丽华李垚
Owner HARBIN INST OF TECH
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