Hansenula polymorpha-based method for producing D-arabitol
A technology of Hansenula polymorpha and arabinitol, which is applied in the field of Hansenula polymorpha DL-1 fermentation to produce D-arabitol, which can solve the problems of high sterility requirements, high separation requirements, and restrictions on the development of biological sugar alcohols, etc. problems, to achieve the effect of easy operation, increased output and low cost
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Embodiment 134
[0043] Example 134 ℃ constant temperature fermentation
[0044] Pick a single colony after activating the acclimatized strains preserved on the slant for 3 hours at 37°C, and inoculate it into a test tube containing 5 mL of seed medium for cultivation (cultivation conditions: 160 r / min, shake culture at 37°C for 24 hours) to obtain fermentation Seed culture fluid. The fermented seed culture solution was inoculated into a 500mL Erlenmeyer flask containing 100mL fermentation medium at an inoculum size of 5% (v / v) for fermentation (160r / min, 144h shaking at 34°C) to obtain a fermentation solution. The DCW in the fermentation broth was 5.09g / L, and the extracellular production of D-arabitol was 95.03g / L.
Embodiment 237
[0045] Example 237 ℃ constant temperature fermentation
[0046] During the fermentation process, the temperature was 37° C., and the others were the same as in Example 1. The DCW in the fermentation broth was 5.28 g / L, and the extracellular production of D-arabitol was 88.06 g / L.
Embodiment 348
[0047] Example 348 ° C constant temperature fermentation
[0048] During the fermentation process, the temperature was 48° C., and the others were the same as in Example 1. The DCW in the fermentation broth was 3.21 g / L, and the extracellular production of D-arabitol was 37.23 g / L.
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