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D-arabitol production method based on Hansenula polymorpha

A technology of Hansenula polymorpha and arabinitol, applied in the field of Hansenula polymorpha DL-1 fermentation to produce D-arabitol, which can solve the problems of high sterility requirements, restrictions on the development of biological sugar alcohols, and high separation requirements , to achieve the effect of easy operation, low cost and increased output

Active Publication Date: 2016-08-17
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still shortcomings such as high sterility requirements and high separation requirements in the later stage, which also limit the development of bio-sugar alcohols
In addition, the current biotransformation method does not make full use of the influence of changes in fermentation conditions on the metabolic mechanism of yeast cells to achieve the purpose of increasing the production of D-arabitol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 134

[0043] Example 134 ℃ constant temperature fermentation

[0044] Pick a single colony after activating the acclimatized strains preserved on the slant for 3 hours at 37°C, and inoculate it into a test tube containing 5 mL of seed medium for cultivation (cultivation conditions: 160 r / min, shake culture at 37°C for 24 hours) to obtain fermentation Seed culture fluid. The fermented seed culture solution was inoculated into a 500mL Erlenmeyer flask containing 100mL fermentation medium at an inoculum size of 5% (v / v) for fermentation (160r / min, 144h shaking at 34°C) to obtain a fermentation solution. The DCW in the fermentation broth was 5.09g / L, and the extracellular production of D-arabitol was 95.03g / L.

Embodiment 237

[0045] Example 237 ℃ constant temperature fermentation

[0046] During the fermentation process, the temperature was 37° C., and the others were the same as in Example 1. The DCW in the fermentation broth was 5.28 g / L, and the extracellular production of D-arabitol was 88.06 g / L.

Embodiment 348

[0047] Example 348 ° C constant temperature fermentation

[0048] During the fermentation process, the temperature was 48° C., and the others were the same as in Example 1. The DCW in the fermentation broth was 3.21 g / L, and the extracellular production of D-arabitol was 37.23 g / L.

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Abstract

The invention provides a hansenula polymorpha-based method for producing D-arabitol. The method comprises the following steps: carrying out fermented seed cultivation on hansenula polymorpha after domestication under a hyperosmosis condition, and then inoculating into a fermentation medium; firstly, cultivating at 30-38 DEG C for 18-24 hours, and then continuing to cultivate at 40-49 DEG C for 18-24 hours; and finally continuing to cultivate at 28-38 DEG C for 74-96 hours. The ability of the hansenula polymorpha for producing the D-arabitol is improved by comprehensive strain domestication and fermentation temperature regulation technologies, and the dry cell weight and the yield of the D-arabitol respectively can achieve 5.26g / L and 114.92g / L. According to the method, the yield of the D-arabitol produced by hansenula polymorpha fermentation can be increased; in addition, the method provided by the invention is easy to operate, relatively low in cost, and easy to apply in the fermentation method for producing the D-arabitol.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for producing D-arabitol by fermentation of Hansenula polymorpha (H. polymorpha) DL-1. Background technique [0002] D-arabitol is a five-carbon polysaccharide alcohol. Its molecular formula is C 5 h 12 O 5 , the relative molecular weight is 152.12, the melting point is 103°C, the optical rotation is 130°C, it is a white columnar crystal at room temperature, easily soluble in water, but insoluble in ether. Polysaccharide alcohols refer to a class of polyols produced by hydrogenation of the carbonyl groups of sugars. D-arabitol has been widely used in medicine, food, chemical industry and other fields. D-arabitol mainly exists in mushrooms and lichens, but the content is very low, which cannot meet the growing market demand. [0003] At present, the production method of D-arabitol is mainly through chemical synthesis, that is, catalytic hydrogenation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/78
Inventor 钱卫东宁肖肖赵德志蔡长龙毛培宏王婷
Owner SHAANXI UNIV OF SCI & TECH
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