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Molecular markers for assistant selection of resistance gene Ph of tobacco black shank and application thereof

A molecular marker, tobacco black shank technology, applied in the field of plant molecular genetics and tobacco germplasm innovation, to achieve the effect of reducing production costs, reducing economic losses, and simple and quick operation

Active Publication Date: 2014-08-20
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no tobacco black shank resistance gene at home and abroad Ph Reports of Molecular Markers Tightly Linked on Both Sides

Method used

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  • Molecular markers for assistant selection of resistance gene Ph of tobacco black shank and application thereof
  • Molecular markers for assistant selection of resistance gene Ph of tobacco black shank and application thereof
  • Molecular markers for assistant selection of resistance gene Ph of tobacco black shank and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Verification of molecular marker TM29 detection Ph genetic accuracy

[0054] A selection of 14 materials belonging to 5 different tobacco types: N. plumbaginifolia (wild smoke, incl. Ph gene); Coker371-Gold (cured tobacco, containing Ph gene); RBST (cured tobacco, containing Ph gene); F 1 (RBST×Red Flower Gold Dollars, including Ph gene); Honghua Dajinyuan (cured tobacco, excluding Ph gene); Yunyan 85 (cured tobacco, excluding Ph gene); Yunyan 87 (cured tobacco, excluding Ph gene); K326 (cured tobacco, excluding Ph gene); Hicks (cured tobacco, without Ph gene ); Danyu No. 2 (flue-cured tobacco, excluding Ph gene); China Tobacco 100 (flue-cured tobacco, excluding Ph gene); Florida301 (cigar, excluding Ph gene); Beinhart-1000 (air-cured tobacco, excluding Ph Gene); Samsun (oriental tobacco, free of Ph Gene).

[0055] The DNA of 14 materials was extracted, and a PCR reaction system was established. The total volume of the PCR reaction system was 2...

Embodiment 2

[0057] Example 2: Verification of molecular marker TM50 detection Ph genetic accuracy

[0058] A selection of 14 materials belonging to 5 different tobacco types: N. plumbaginifolia (wild smoke, incl. Ph gene); Coker371-Gold (cured tobacco, containing Ph gene); RBST (cured tobacco, containing Ph gene); F 1 (RBST×Red Flower Gold Dollars, including Ph gene); Honghua Dajinyuan (cured tobacco, excluding Ph gene); Yunyan 85 (cured tobacco, excluding Ph gene); Yunyan 87 (cured tobacco, excluding Ph gene); K326 (cured tobacco, excluding Ph gene); Hicks (cured tobacco, without Ph gene ); Danyu No. 2 (flue-cured tobacco, excluding Ph gene); China Tobacco 100 (flue-cured tobacco, excluding Ph gene); Florida301 (cigar, excluding Ph gene); Beinhart-1000 (air-cured tobacco, excluding Ph Gene); Samsun (oriental tobacco, free of Ph Gene).

[0059] Extract DNA from 14 materials and establish a PCR reaction system with a total volume of 20 μL, including 0.8 μmol / L each of the ...

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Abstract

The invention provides molecular markers for assistant selection of a resistance gene (Ph) of tobacco black shank and an application thereof. The molecular markers provided by the invention are TM29 and TM50 which are respectively located on both sides of the gene (Ph) on a 20th gene linkage group and are tightly interlocked with the gene (Ph). The genetic distance from the molecular marker TM29 to the gene (Ph) is 0.091cM and that from the molecular marker TM50 to the gene (Ph) is 0.148cM. The invention further provides a molecular marking method for detecting the resistance gene (Ph) of tobacco black shank by using the molecular markers as well as the application of the molecular markers in assistant breeding of tobacco black shank and positioning of the resistance gene (Ph) of tobacco black shank. The molecular markers for assistant selection provided by the invention is clear in selection target and cost-saving, remarkably improves the selection efficiency, not only provides a resistant resource screening method to breeding of tobacco black shank, but also gets close to the gene (Ph) through a genome walking method, thereby laying a foundation for cloning of the gene (Ph).

Description

technical field [0001] The invention belongs to the field of plant molecular genetics and tobacco germplasm innovation technology, and specifically relates to a tobacco black shank resistance gene Ph Molecular markers for assisted selection and their applications. Background technique [0002] Tobacco black shank is caused by the fungus Tobacco black shank ( Phytophthora parasitica var. nicotianae ) caused by a soil-borne fungal disease is one of the main diseases that affect the yield and quality of tobacco. After dyeing, the whole tobacco plant often dies, causing huge economic losses (Wang Wanneng, Xiao Chonggang. Comprehensive Control and Research Progress of Tobacco Black Leg Disease. Guangxi Agricultural Sciences, 2003(2):42-43.). One of the safest and most cost-effective measures to control tobacco black shank is to breed disease-resistant varieties. [0003] There are four main sources of resistance to tobacco black shank, namely the cigar variety Florida301, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 童治军肖炳光李永平陈学军方敦煌
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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