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The construction method of the fingerprint of traditional Chinese medicine Luo Xianzi

The technology of a fingerprint spectrum and a construction method is applied in the field of the construction of the fingerprint spectrum of the traditional Chinese medicine Luo Xianzi, which can solve the problems of no traditional Chinese medicine Luo Xianzi fingerprint and report, and achieves good stability and reproducibility, strong operability, and simple steps. Effect

Inactive Publication Date: 2015-08-26
GUANGDONG PHARMA UNIV
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AI Technical Summary

Problems solved by technology

So far, there is no report on the fingerprint of the traditional Chinese medicine Luo Xianzi

Method used

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  • The construction method of the fingerprint of traditional Chinese medicine Luo Xianzi
  • The construction method of the fingerprint of traditional Chinese medicine Luo Xianzi

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Experimental program
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Effect test

Embodiment

[0043] 1. Instruments and reagents:

[0044] Waters high-performance liquid chromatography was used; there were 10 batches of traditional Chinese medicine Luoxianzi, which were cultivated by the Institute of Bioactive Substances of Guangdong Pharmaceutical University (the sample collection dates of the 10 batches were 2012-11-20, 2012-12-16, 2012-12-31, 2013-01-18, 2013-01-25, 2013-03-27, 2013-04-19, 2013-05-10, 2013-06-19, 2013-07-08 Fairy); other reagents were chromatographically pure or analytically pure, and were directly purchased commercially.

[0045] Routine solution preparation:

[0046] (1) 0.05mol / L sodium acetate buffer solution (containing 10ml / L N,N-dimethylformamide): Weigh 4.1g sodium acetate, add 700ml pure water to dissolve, adjust pH to 6.5 with acetic acid, add water to 1L, Add 10ml of N,N-dimethylformamide, and filter through a 0.45μm filter;

[0047] (2) 10ml / L DNFB (2,4 dinitrofluorobenzene) solution: take 1.0ml DNFB, add acetonitrile to make up to 10...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine analysis and discloses a construction method of a traditional Chinese medicine musca domestica fingerprint. The construction method comprises traditional Chinese medicine musca domestica hydrolysis, derivatization, and high performance liquid chromatography condition selection and determination, realizes traditional Chinese medicine musca domestica fingerprint construction, ensures retention time of 0-35min and produces 24 common characteristic peaks and is suitable for further true-false identification and quality classification of the traditional Chinese medicine musca domestica. The construction method of the traditional Chinese medicine musca domestica fingerprint has simple processes, a low cost, good stability and reappearance, good operationality and high precision.

Description

technical field [0001] The invention belongs to the technical field of analysis of traditional Chinese medicinal materials, and in particular relates to a method for constructing fingerprints of the traditional Chinese medicine Luoxianzi. Background technique [0002] Chinese medicine Luo Xianzi ( Musca domestica ), commonly known as maggots, also known as grain worms, grain worms, water fairies, etc., commonly known as Luo fairy in Guangdong. It is recorded in the fortieth volume of the Department of Insects in "Compendium of Materia Medica". The insect is cold in nature and non-toxic. It enters the spleen and stomach meridians and can treat infectious surgical diseases such as ecthyma, lip furuncle and carbuncle. In recent years, studies have found that Luoxianzi is rich in active ingredients such as lectin, lysozyme, and antibacterial protein, and has antibacterial, anti-inflammatory, anti-oxidation, and anti-tumor activities. [0003] Traditional Chinese medicine finge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 朱家勇褚夫江金小宝李小波梅寒芳吴玉萍
Owner GUANGDONG PHARMA UNIV
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