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Application of protein arginine methyltransferase 7 in cancer cell metastasis

An arginine methyl, protein technology, applied in tumor/cancer cells, cells modified by introducing foreign genetic material, animal cells, etc.

Inactive Publication Date: 2014-09-10
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the function of PRMT7 is still very limited. PRMT7 is involved in the maintenance of pluripotency of mouse embryonic stem cells and sperm cells

Method used

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  • Application of protein arginine methyltransferase 7 in cancer cell metastasis
  • Application of protein arginine methyltransferase 7 in cancer cell metastasis
  • Application of protein arginine methyltransferase 7 in cancer cell metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1, PRMT7 is highly expressed in human malignant breast cancer samples

[0107] 1. Immunohistochemistry of human malignant breast cancer samples

[0108] (1) Dewaxing and hydrating human malignant breast cancer sample tissue into sections, washing with PBS 3 times, 5 minutes each time;

[0109] (2) Antigen thermal retrieval: put tissue slices into 95°C 10mM citrate buffer (pH6.0) and heat for 15min;

[0110] (3) Use 3% H by volume 2 o 2 Incubate tissue sections with aqueous solution at room temperature for 5-10 minutes to eliminate the activity of endogenous peroxidase;

[0111] (4) Wash 3 times with PBS, 5 minutes each time;

[0112] (5) Add blocking solution dropwise and incubate at room temperature for 5-10 minutes;

[0113] (6) Add an appropriate amount of primary antibody working solution of rabbit-derived anti-PRMT7 immunohistochemical antibody (dilute the primary antibody 1:50 times with antibody diluent), incubate at room temperature for 1 hour, wash w...

Embodiment 2

[0263] Example 2, PRMT7 promotes distant metastasis of breast cancer

[0264] 1. Overexpress PRMT7 in the breast cancer cell line MCF-7 (method is the same as the process of obtaining the cell line stably transfected with PRMT7 in step 4 in Example 1, replace the target cells with MCF-7 and PRMT7-MSCV2. 2-IRES-GFP was replaced by PRMT7-PWPXLD) to obtain the stably transfected PRMT7-MCF-7 cell line, and the same method was used to obtain control cells (only PRMT7-PWPXLD was replaced by the control plasmid PWPXLD).

[0265] Two kinds of cells, the stably transfected PRMT7-MCF-7 cell line and the control group cells, were injected into the tail vein of BALB / c nude mice (purchased from Beijing Huafukang Biotechnology Co., Ltd.) respectively (the number of injected cells was equal to 2×10 6 cells) to obtain mice in the experimental group and mice in the control group, respectively. After 2 months, the mice were tested using small animal live imaging technology, and the results we...

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Abstract

The invention discloses an application of protein arginine methyltransferase 7 in cancer cell metastasis. The invention also discloses an application of the protein arginine methyltransferase 7 in preparation of a product for promoting epithelial-mesenchymal transition. The invention proves that PRMT7 (protein arginine methyltransferase 7) has extremely high expression in breast cancer tissues and can induce breast cancer cells to generate EMT (Epithelial Mesenchymal Transition) and promote intrusion and migration of the breast cancer cells. The EMT can be inhibited by interfering the PRMT7, the intrusion and migration capacity of the breast cancer cells can be reduced, and the PRMT7 can serve as a target to be applied to cancer therapy.

Description

technical field [0001] The invention relates to the application of protein arginine methyltransferase 7 in cancer cell metastasis, and belongs to the field of biopharmaceuticals. Background technique [0002] Breast cancer is a public health disease that has been widely concerned and valued. Its incidence group is mainly women. Of course, a small number of men are also found to be the incidence group. Breast cancer is the second most common cancer in women and has a high mortality rate. and received the attention of the general public. Breast cancer is one of the most common malignant tumors in women. [0003] Protein arginine methyltransferase 7 (PRMT7), a domain with two AdoMet binding sites, has different enzymatic activities for different substrates, and can catalyze the monomethylation of fibrin. PRMT7 can affect drug-induced sensitivity and interact with CTCFL to mediate gene imprinting in male sperm. After analyzing the metastasis-related chromosomal regions of 120...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/10A61K45/00A61K48/00A61P35/00A61P35/04
Inventor 陆军张瑜姚若斯姜浩李晓雪黄百渠
Owner NORTHEAST NORMAL UNIVERSITY
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