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Fast expanding propagation method for mycorrhiza fungus strain

A technology for mycorrhizal fungi and strains, applied in the field of microorganisms, can solve the problems of decreased strain purity, poor water holding capacity, time-consuming and water-consuming, etc., and achieve the effects of reducing the direct contact area, speeding up the growth time, and avoiding the contamination of miscellaneous bacteria.

Inactive Publication Date: 2014-09-17
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the conventional mycorrhizal fungus propagation technology has the following disadvantages: (1) it takes 4-5 months to complete a propagation cycle, which takes a long time; (2) the substrate used for propagation is mainly sandstone, which holds water Poor performance, it needs to be watered every 2-3 days, which is time-consuming and water-consuming; (3) The substrate is easily polluted by bacteria exposed to the air during the propagation process, resulting in a decrease in the purity of the strains and even failure in propagation

Method used

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  • Fast expanding propagation method for mycorrhiza fungus strain
  • Fast expanding propagation method for mycorrhiza fungus strain
  • Fast expanding propagation method for mycorrhiza fungus strain

Examples

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0018] figure 1 After completing the weighing of the sterilized sand and gravel matrix, inoculation of bacteria, sowing of host plants (sorghum and corn) and watering and sand covering, the flower pots were moved into the greenhouse, and finally a layer of plastic wrap was placed on the flower pots to Water retention and heat preservation.

[0019] The specific operation steps are: (1) Put the sterilized matrix (sandstone) into the flower pot to 3 / 5, add 10-15g of inoculation agent and spread it on the matrix, and then cover a layer of sterilized matrix to the flower pot At 4 / 5 places, after watering thoroughly, sow 15-20 host plant (sorghum, corn) seeds per pot, then cover with 0.5-1 cm of sterilized sand, and finally move the flower pots into the greenhouse and cover them with a fresh-keeping layer Use film to keep water and keep warm, promote rapid...

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Abstract

The invention discloses a fast expanding propagation method for a mycorrhiza fungus strain. The method comprises the steps of matrix preparing, strain inoculating, host sowing, culturing managing as well as harvesting and preserving. Needed materials of the method are common, the operation process is simple, timesaving and laborsaving, the expanding-propagated strain can achieve the effect, which can be achieved in five months by the conventional expanding propagation technology, in just three months, and compared with that of the conventional expanding propagation technology, time of the expanded propagation technology is shortened by two or even thee months; the fast expanding propagation method can be widely applied in the fields of biological control, ecological environment protection and modernized high-tech agriculture while the strain fungus expanding-propagated by the invention can be used for a test.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for quickly and efficiently multiplying mycorrhizal fungi by soil beneficial microorganisms. Background technique [0002] At present, the conventional mycorrhizal fungus propagation technology has the following disadvantages: (1) it takes 4-5 months to complete a propagation cycle, which takes a long time; (2) the substrate used for propagation is mainly sandstone, which holds water Poor performance, it needs to be watered every 2-3 days, which is time-consuming and water-consuming; (3) The substrate is easily polluted by bacteria exposed to the air during the propagation process, resulting in a decrease in the purity of the strains and even failure in propagation. Contents of the invention [0003] In order to quickly and efficiently propagate mycorrhizal fungi and meet the needs of experiments and practical use, the invention provides a time-saving, labor-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
Inventor 张峰段延玉南志标高鹏闫飞扬李芳
Owner LANZHOU UNIVERSITY
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