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Preparation method of high-purity salvianolic acid B

A technology of salvianolic acid and salvia miltiorrhiza, applied in the field of separation and purification of active components of traditional Chinese medicine, can solve problems such as difficulty, increase process steps, hinder the development of salvianolic acid B injection, and achieve low price, low cost, and environmental benefits the protective effect of

Inactive Publication Date: 2014-10-01
ZHEJIANG YONGNING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the salvianolic acid B obtained by this method has a high purity, it needs to go through a one-step microbial degradation process, which increases the steps of the process.
[0006] At present, it is still quite difficult to industrially prepare high-purity salvianolic acid B (purity ≥ 99%, HPLC), which seriously hinders the development of salvianolic acid B injection

Method used

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  • Preparation method of high-purity salvianolic acid B
  • Preparation method of high-purity salvianolic acid B
  • Preparation method of high-purity salvianolic acid B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1. Add 100 g of Danshen medicinal material, add 1 L of water, stir and extract twice at 80° C. for 1 hour each time, filter, combine the two filtrates, and concentrate the filtrate to 200 mL.

[0041] Step 2, adjust the pH of the extract to 2, pass through a 100mL macroporous adsorption resin XAD2 column (height-to-diameter ratio 8:1), elute with 50% ethanol, collect the 50% ethanol eluate step by step, detect by HPLC, and the combined purity exceeds 80% %sample.

[0042] Step 3. Concentrate the above 80% salvianolic acid B ethanol solution with a purity greater than 80% to no alcohol smell, adjust to pH7, pass through 100mL microsphere resin microsphere No. 1 resin column (height-to-diameter ratio 8:1), and elute with water. Collect in steps, detect by HPLC, combine the samples with a purity exceeding 99%, and purify the samples with a purity of less than 99% by microsphere resin again. Freeze-dry the salvianolic acid B sample with a purity of 99%, and finally ob...

Embodiment 2

[0044] In Step 1 of Example 1, the extraction solvent of Salvia miltiorrhiza was replaced with 10% ethanol, and in Step 2, the macroporous adsorption resin was replaced with XAD4.

Embodiment 3

[0046] In Step 1 of Example 1, the extraction solvent of Salvia miltiorrhiza was replaced with 80% ethanol, and in Step 2, the macroporous adsorption resin was replaced with XAD16HP.

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Abstract

The invention discloses a method for separating and purifying salvianolic acid B from danshen root. The method comprises the following steps: performing solvent extraction on a danshen root medicinal material, and concentrating an extracting solution to recycle a solvent; performing one-step macro-porous adsorption resin primary purification on danshen root extract subjected to solvent recovery, and performing solvent elution to obtain a salvianolic acid B sample of which the purity is more than 80%; performing one-step microsphere resin purification on the sample again to finally obtain salvianolic acid B of which the purity is more than 99%. A process is simple in operation and easy in industrial amplification, is suitable for preparing the salvianolic acid B high-purity sample, and can ensure that the purity of final salvianolic acid B is more than 99%, and the yield of salvianolic acid B is more than 1.5% (calculated according to dry medicinal materials).

Description

technical field [0001] The invention relates to a method for separating and purifying active components of traditional Chinese medicines, in particular to a method for preparing salvianolic acid B from salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza is derived from the dry root and rhizome of Salvia miltiorrhiza. Danshen is widely used in the treatment of cardiovascular and cerebrovascular diseases, liver diseases, kidney diseases, diabetic complications, and nervous system diseases. The main active components in Danshen include fat-soluble phenanthrenequinones (represented by tanshinone IIA) and water-soluble salvianolic acids (represented by salvianolic acid B). Since the use of traditional Chinese medicine is mainly decoction, the water-soluble components of Danshen have attracted everyone's attention. Salvianolic acid B is the water-soluble group of Salvia miltiorrhiza with the highest content [0003] It is reported in the literature that it ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/86
CPCC07D307/86
Inventor 施梅仙
Owner ZHEJIANG YONGNING PHARMA
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