Method for rapid artificial inoculation in-vitro identification of Batryris elliptica Cocke resistance

Abstract

A method for the rapid artificial inoculation in-vitro identification of Batryris elliptica Cocke resistance comprises the following steps: culturing Batryris elliptica Cocke in a culturing container by using a potato and oat starch culturing medium, disinfecting by using an autoclave, and standing for cooling to prepare a culturing medium; acquisition of lily to be measured: taking healthy initial-squaring-stage young lily plants which are not infected with Batryris elliptica Cocke pathogens and must have 6-9 healthy blades, and respectively taking complete lily blades from an upper position, a middle position and a lower position; pathogen acquisition: taking typical infected lily blades, separating and purifying pathogens, identifying the pathogens as Batryris elliptica Cocke, culuring the pathogens on the potato and oat starch culturing medium for 7d, and inoculating the healthy lily blades; inoculating by adopting a mycelium block inoculation process, a spore spray inoculation process or a mechanical inoculation process; wrapping petiole wounds of the pathogen inoculated lily blades with wet cotton, putting the inoculated lily blades in a culture dish, and sprinkling a proper amount of sterile water to carry out moisture retention culture; and investigating the disease infection conditions of the blades at the 3th day, the 5th day, the 7th and the 9th day, observing, and identifying. The Batryris elliptica Cocke resistance of different varieties of lily can be accurately and efficiently identified under controllable conditions through the method.

Description

technical field

[0001] The invention belongs to the technical field of identification of flower disease resistance, and in particular relates to a method for rapid artificial inoculation and in vitro identification of lily botrytis resistance. Background technique

[0002] lily( Lilium spp. ) is a perennial herbaceous bulbous plant of the genus Lily of the family Liliaceae. It is mainly distributed in the temperate regions of the northern hemisphere such as eastern Asia, Europe, and North America. Hundreds of species have been found in the world, of which 55 species are produced in China. China is its main origin . Lily is one of the most popular fresh cut flowers in the world because of its beautiful appearance and extraordinary bearing, in addition to its traditional medicinal, edible and essence extraction. Lily Botrytis ( Botrytis cinerea ) is the main disease of the aboveground part of lily, and it is one of the important factors that inhibit the production of lily....