Method for rapid artificial inoculation in-vitro identification of Batryris elliptica Cocke resistance

A technology of artificial inoculation and gray mold, applied in the direction of microorganism-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of slow progress in the breeding of lily resistance to gray mold

Inactive Publication Date: 2014-10-08
SHENYANG AGRI UNIV
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  • Summary
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AI Technical Summary

Problems solved by technology

[0005] For a long time, the breeding of lily for resistance to Botrytis cinerea has progressed slowly due to the lack of screening of resistant materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] The successful in vitro rapid inoculation identified the resistance of the lily variety 'Xin Tie Pao' to lily gray mold, and determined that the lily variety 'Xin Tie Pao' was susceptible to lily gray mold.

[0023] The specific implementation method is as follows:

[0024] A. Culture vessel preparation.

[0025] Use a 9cm flat culture dish as an in vitro culture container. Wash it with tap water first, then rinse it with distilled water 3 times, and then place 2-3 pieces of filter paper in a petri dish.

[0026] B. Culture medium preparation.

[0027] 1. The basic components of the medium: agar, potato, oat, starch.

[0028] The specific content is: agar 20g / L, potato 200g / L, oat 20g / L, starch 20g / L.

[0029] 2. Preparation method of culture medium

[0030] Weigh 20g of oats and wrap them in gauze, weigh 200g of peeled potatoes and chop them up, add them to 1000mL of distilled water and boil for 20 minutes, then filter with gauze. Add water to 1000mL, add 20g of ...

Embodiment 2

[0049] Successful in vitro inoculation identified the resistance of the lily variety 'Minjiang' to lily gray mold, and identified the resistance of the lily variety 'Minjiang' to lily gray mold.

[0050] The specific method of implementation is as follows:

[0051]A. In implementation, culture container preparation, culture medium preparation, lily leaf collection to be tested, pathogenic bacteria collection, pathogenic bacteria inoculation, concrete culture method are the same as example 1, difference is, when cultivating the 5th day, with the friction inoculation method inoculation The leaves of lily cultivar 'Minjiang' to be tested had fewer diseased spots, and the area of ​​diseased spots accounted for less than 5% of the total leaf area. Moreover, the leaves inoculated by the mycelium block inoculation method and the spore liquid spray inoculation method have no disease spots.

[0052] The identification results showed that the lily variety 'Minjiang' was a disease-resis...

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PUM

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Abstract

A method for the rapid artificial inoculation in-vitro identification of Batryris elliptica Cocke resistance comprises the following steps: culturing Batryris elliptica Cocke in a culturing container by using a potato and oat starch culturing medium, disinfecting by using an autoclave, and standing for cooling to prepare a culturing medium; acquisition of lily to be measured: taking healthy initial-squaring-stage young lily plants which are not infected with Batryris elliptica Cocke pathogens and must have 6-9 healthy blades, and respectively taking complete lily blades from an upper position, a middle position and a lower position; pathogen acquisition: taking typical infected lily blades, separating and purifying pathogens, identifying the pathogens as Batryris elliptica Cocke, culuring the pathogens on the potato and oat starch culturing medium for 7d, and inoculating the healthy lily blades; inoculating by adopting a mycelium block inoculation process, a spore spray inoculation process or a mechanical inoculation process; wrapping petiole wounds of the pathogen inoculated lily blades with wet cotton, putting the inoculated lily blades in a culture dish, and sprinkling a proper amount of sterile water to carry out moisture retention culture; and investigating the disease infection conditions of the blades at the 3th day, the 5th day, the 7th and the 9th day, observing, and identifying. The Batryris elliptica Cocke resistance of different varieties of lily can be accurately and efficiently identified under controllable conditions through the method.

Description

technical field [0001] The invention belongs to the technical field of identification of flower disease resistance, and in particular relates to a method for rapid artificial inoculation and in vitro identification of lily botrytis resistance. Background technique [0002] lily( Lilium spp. ) is a perennial herbaceous bulbous plant of the genus Lily of the family Liliaceae. It is mainly distributed in the temperate regions of the northern hemisphere such as eastern Asia, Europe, and North America. Hundreds of species have been found in the world, of which 55 species are produced in China. China is its main origin . Lily is one of the most popular fresh cut flowers in the world because of its beautiful appearance and extraordinary bearing, in addition to its traditional medicinal, edible and essence extraction. Lily Botrytis ( Botrytis cinerea ) is the main disease of the aboveground part of lily, and it is one of the important factors that inhibit the production of lily....

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
Inventor 陈丽静杨妮娜孙少坤马镝孙红梅苏瑛郭志富马慧
Owner SHENYANG AGRI UNIV
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